Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1350] to JAB1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
製品名Anti-JAB1 antibody [EPR1350] - BSA and Azide free
JAB1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR1350] to JAB1 - BSA and Azide free
アプリケーション適用あり: ICC/IF, IHC-P, WB, IPmore details
適用なし: Flow Cyt
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human JAB1 aa 1-100. The exact sequence is proprietary.
Ab247980 is the carrier-free version of ab124720. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247980 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 6.45 x 10 -11 M Learn more about KD
Concentration information loading...
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- SCF Complex E3 Ligase
Our Abpromise guarantee covers the use of ab247980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.|
|IP||Use at an assay dependent concentration.|
機能Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
配列類似性Belongs to the peptidase M67A family. CSN5 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
ドメインThe JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
- Information by UniProt
- 38 kDa Mov34 homolog antibody
- COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
- COP9 constitutive photomorphogenic homolog subunit 5 antibody
All lanes : Anti-JAB1 antibody [EPR1350] (ab124720) at 1/1000 dilution
Lane 1 : NIH 3T3 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
This data was developed using ab124720, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.