Anti-JAB1 抗体 (ab12323)
Key features and details
- Rabbit polyclonal to JAB1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-JAB1 antibody
JAB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to JAB1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis
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免疫原
Immunogen was a synthetic peptide, which represented a c-terminal domain of human JAB1 (LocusLink ID 10987).
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特記事項
Centrifuge tube to remove product from lid.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12323の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
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| IHC-P |
1/250 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB | (2) |
1/5000 - 1/25000. Detects a band of approximately 33, 36 kDa (predicted molecular weight: 40 kDa).
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| 特記事項 |
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IHC-P
1/250 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/25000. Detects a band of approximately 33, 36 kDa (predicted molecular weight: 40 kDa). |
ターゲット情報
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機能
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. -
配列類似性
Belongs to the peptidase M67A family. CSN5 subfamily.
Contains 1 MPN (JAB/Mov34) domain. -
ドメイン
The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10987 Human
- Entrez Gene: 26754 Mouse
- Entrez Gene: 312916 Rat
- Entrez Gene: 444720 Xenopus laevis
- Entrez Gene: 393698 Zebrafish
- Omim: 604850 Human
- SwissProt: Q92905 Human
- SwissProt: O35864 Mouse
see all -
別名
- 38 kDa Mov34 homolog antibody
- COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
- COP9 constitutive photomorphogenic homolog subunit 5 antibody
see all
画像
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Western blot - Anti-JAB1 antibody (ab12323)This image is courtesy of an anonymous AbreviewAll lanes : Anti-JAB1 antibody (ab12323) at 1/2500 dilution
Lane 1 : HEK293 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lane 3 : U2OS whole cell lysate
Lane 4 : Mouse embryonic fibroblast whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAB1 antibody (ab12323)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling JAB1 with ab12323 at 1/500 (2µg/ml). Detection: DAB. -
Western blot - Anti-JAB1 antibody (ab12323)Detection of Human CSN5 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure. Detection of Human CSN5 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab12323 は 6 報の論文で使用されています。
- Keuss MJ et al. Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death. EMBO J 38:N/A (2019). PubMed: 30804002
- Esquerdo M et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . PubMed: 26934049
- Eckle T et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). PubMed: 22504483
- Chua YS et al. Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo. PLoS One 6:e16071 (2011). WB ; Human . PubMed: 21249194
- Leck YC et al. Biochemical and cellular effects of inhibiting Nedd8 conjugation. Biochem Biophys Res Commun 398:588-93 (2010). WB ; Human . PubMed: 20603103
- Chew EH & Hagen T Substrate-mediated regulation of cullin neddylation. J Biol Chem 282:17032-40 (2007). WB ; Human . PubMed: 17439941