Anti-ITPR2 抗体 (ab5805)
Key features and details
- Rabbit polyclonal to ITPR2
- Suitable for: ICC, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ITPR2 antibody
ITPR2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ITPR2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-Frmore details
適用なし: WB -
種交差性
交差種: Rat, Human
交差が予測される動物種: Cow
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免疫原
Synthetic peptide corresponding to Rat ITPR2 aa 317-334.
Sequence:ELNPDYRDAQNEGKTVRD
(Peptide available asab5842) -
ポジティブ･コントロール
- IHC-Fr: Rat brain tissue. ICC: SH-SY5Y cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5805の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 2 µg/ml.
|
|
| IHC-Fr |
1/100.
|
| 特記事項 |
|---|
|
ICC
Use a concentration of 2 µg/ml. |
|
IHC-Fr
1/100. |
ターゲット情報
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機能
Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA. -
組織特異性
Isoform Short is found in skeletal muscle and heart. -
関連疾患
Anhidrosis, isolated, with normal sweat glands -
配列類似性
Belongs to the InsP3 receptor family.
Contains 5 MIR domains. -
ドメイン
The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. -
翻訳後修飾
Phosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281878 Cow
- Entrez Gene: 3709 Human
- Entrez Gene: 81678 Rat
- Omim: 600144 Human
- SwissProt: Q8WN96 Cow
- SwissProt: Q14571 Human
- SwissProt: P29995 Rat
- Unigene: 512235 Human
see all -
別名
- 4 antibody
- 5-trisphosphate receptor antibody
- Inositol 1 4 5 trisphosphate receptor type 2 antibody
see all
画像
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Immunocytochemistry - Anti-ITPR2 antibody (ab5805)
4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling ITPR2 using ab5805 at 2 μg/ml in 0.1% BSAfor 3 hours at RT, followed by a Goat Anti-Rabbit (H&L) AlexaFluor® secondary antibody (Green (a)). The nuclear counter stain is DAPI (Blue (b)). F-actin is stained with AlexaFluor® 555-Rhodamine (Red (c)). Panel D shows the merged image. Panel (e) is a secondary antibody only control.
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Immunohistochemistry (Frozen sections) - Anti-ITPR2 antibody (ab5805)Shows immunolocalization of IP3R-II in rat brain using ab5805.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab5805 は 4 報の論文で使用されています。
- Jaiswal PK et al. Biochemical characteristics of the chondrocyte-enriched SNORC protein and its transcriptional regulation by SOX9. Sci Rep 10:7790 (2020). PubMed: 32385306
- Causton B et al. CARMA3 Mediates Allergic Lung Inflammation in Response to Alternaria alternata. Am J Respir Cell Mol Biol 59:684-694 (2018). PubMed: 29958012
- Kapoor N et al. Regulation of calcium clock-mediated pacemaking by inositol-1,4,5-trisphosphate receptors in mouse sinoatrial nodal cells. J Physiol 593:2649-63 (2015). PubMed: 25903031
- Okamoto Y et al. Arrhythmogenic coupling between the Na+ -Ca2+ exchanger and inositol 1,4,5-triphosphate receptor in rat pulmonary vein cardiomyocytes. J Mol Cell Cardiol 52:988-97 (2012). ICC/IF ; Rat . PubMed: 22285480
