Anti-Isopeptide 抗体 [81D1C2] (ab422)
Key features and details
- Mouse monoclonal [81D1C2] to Isopeptide
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-Isopeptide antibody [81D1C2] -
製品の詳細
Mouse monoclonal [81D1C2] to Isopeptide -
由来種
Mouse -
特異性
This antibody reacts with the isopeptide : epsilon-(gamma-glutamyl) lysine. There is no cross reaction with either lysine or glutamine. They are specific to the epsilon-(gamma-glutamyl) lysine bond formed by transglutaminase activity. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic isopeptide.
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特記事項
The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
81D1C2 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/50 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/50 - 1/500. |
ターゲット情報
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別名
- Isopeptide bond antibody
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab422 は 10 報の論文で使用されています。
- Schopfer LM & Lockridge O Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or Asp: Implications for neurotoxicity. J Biol Chem 293:13566-13577 (2018). PubMed: 30006344
- Liu C et al. Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification. Hypertension 63:353-61 (2014). WB . PubMed: 24191290
- Llano E et al. STAG3 is a strong candidate gene for male infertility. Hum Mol Genet 23:3421-31 (2014). PubMed: 24608227
- Nicoll WS et al. Plasmodium falciparum liver stage antigen-1 is cross-linked by tissue transglutaminase. Malar J 10:14 (2011). PubMed: 21255444
- L'Heureux DZ et al. The interaction of angiocidin with tissue transglutaminase. Exp Mol Pathol 88:15-25 (2010). PubMed: 19931242
- Chlenski A et al. SPARC expression is associated with impaired tumor growth, inhibited angiogenesis and changes in the extracellular matrix. Int J Cancer 118:310-6 (2006). PubMed: 16052522
- Mangala LS et al. Tissue transglutaminase-induced alterations in extracellular matrix inhibit tumor invasion. Mol Cancer 4:33 (2005). PubMed: 16153302
- Johnson GV & LeShoure R Immunoblot analysis reveals that isopeptide antibodies do not specifically recognize the epsilon-(gamma-glutamyl)lysine bonds formed by transglutaminase activity. J Neurosci Methods 134:151-8 (2004). PubMed: 15003381
- el Alaoui S et al. Transglutaminase activity and N epsilon (gamma glutamyl) lysine isopeptide levels during cell growth: an enzymic and immunological study. Int J Cancer 48:221-6 (1991). PubMed: 1673452
- Roch AM et al. Differential expression of isopeptide bonds N epsilon (gamma-glutamyl) lysine in benign and malignant human breast lesions: an immunohistochemical study. Int J Cancer 48:215-20 (1991). PubMed: 1673451