Anti-Isopeptide 抗体 [81D1C2] (ab422)

Reviews (1)Q&A (4)References (8)

製品の概要

  • 製品名
    Anti-Isopeptide antibody [81D1C2]
  • 製品の詳細
    Mouse monoclonal [81D1C2] to Isopeptide
  • 由来種
    Mouse
  • 特異性
    This antibody reacts with the isopeptide : epsilon-(gamma-glutamyl) lysine. There is no cross reaction with either lysine or glutamine. They are specific to the epsilon-(gamma-glutamyl) lysine bond formed by transglutaminase activity.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Synthetic isopeptide.

  • 特記事項
    The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    81D1C2
  • ミエローマ
    unknown
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/500.

ターゲット情報

  • 別名
    • Isopeptide bond antibody

プロトコール

参考文献

This product has been referenced in:
  • Liu C  et al. Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification. Hypertension 63:353-61 (2014). WB . Read more (PubMed: 24191290) »
  • Llano E  et al. STAG3 is a strong candidate gene for male infertility. Hum Mol Genet 23:3421-31 (2014). Read more (PubMed: 24608227) »
See all 8 Publications for this product

ab422 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Isopeptide antibody [81D1C2]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Human umbilical artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH=8
Permeabilization
No
Specification
Human umbilical artery
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
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Prof. Jin-Jia Hu

Verified customer

投稿 May 11 2015

Question

Customer has purchased ab422 and needs to know the following information about this antibody: how was the antigen generated? Was cross-linking (such as BSA) involved in generating the peptide? To generate a control, what should the antibody be pre-absorbed with? Any information to help her would be greatly appreciated! Thank you in advance!

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Answer

Antigen: BSA; ab422 is a monoclonal and does not need to be pre-absorbed.

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Question

Hi I`m using your ab 422. Two questions. Do you know if this antibody could be used in flow cytometry analysis? and is there a directly labeled (ex. FITC) version of this antibody? I`m also working with AB8828, is this Ab. suitable for flow studies? Thanks

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Answer

As far as we are aware, ab422 has not yet been tested in in flow cytometry. We do not stock a labelled version of this antibody. ab8828 also has not yet been tested in flow cytometry. If you do test these antibodies in FC, please let us know how you get on by submitting a summary of your results via the reviews section on the datasheets. In return we will forward a reward of your choice -- typically an Amazon gift voucher.

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Question

I am using Ab422, the antibody that recognizes N-epsilon(gamma-glutamyl)Lys. To do appropriate control I need to get N-epsilon(gamma-glutamyl)Lys itself. Do you sell this peptide? Where to get it from?

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Answer

The MAb to isopeptide shows good reactivity on cross linked protein containing isopeptide N-epsilon(gamma-glutamyl)Lys but has weak reactivity on free isopeptide. Therefore commercialy available free isopeptide from Sigma Chemical is not a good positive control.

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Question

Please explain any differences between 153-81D4 and -81D1C2. I wish to perform both western blotting and immunohistochemistry for the isopeptide.

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Answer

The difference between these two antibodies is the isotype: 153-81D4 is an IgM and 153-81D1C2 is an IgG1. They can both be used for both applications, so the choice is yours.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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