Anti-Isopeptide 抗体 [81D1C2] (ab422)
Key features and details
- Mouse monoclonal [81D1C2] to Isopeptide
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-Isopeptide antibody [81D1C2] -
製品の詳細
Mouse monoclonal [81D1C2] to Isopeptide -
由来種
Mouse -
特異性
This antibody reacts with the isopeptide : epsilon-(gamma-glutamyl) lysine. There is no cross reaction with either lysine or glutamine. They are specific to the epsilon-(gamma-glutamyl) lysine bond formed by transglutaminase activity. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic isopeptide.
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特記事項
The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
81D1C2 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/50 - 1/500. |
ターゲット情報
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別名
- Isopeptide bond antibody
参考文献 (10)
ab422 は 10 報の論文で使用されています。
- Schopfer LM & Lockridge O Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or Asp: Implications for neurotoxicity. J Biol Chem 293:13566-13577 (2018). PubMed: 30006344
- Liu C et al. Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification. Hypertension 63:353-61 (2014). WB . PubMed: 24191290
- Llano E et al. STAG3 is a strong candidate gene for male infertility. Hum Mol Genet 23:3421-31 (2014). PubMed: 24608227
- Nicoll WS et al. Plasmodium falciparum liver stage antigen-1 is cross-linked by tissue transglutaminase. Malar J 10:14 (2011). PubMed: 21255444
- L'Heureux DZ et al. The interaction of angiocidin with tissue transglutaminase. Exp Mol Pathol 88:15-25 (2010). PubMed: 19931242
- Chlenski A et al. SPARC expression is associated with impaired tumor growth, inhibited angiogenesis and changes in the extracellular matrix. Int J Cancer 118:310-6 (2006). PubMed: 16052522
- Mangala LS et al. Tissue transglutaminase-induced alterations in extracellular matrix inhibit tumor invasion. Mol Cancer 4:33 (2005). PubMed: 16153302
- Johnson GV & LeShoure R Immunoblot analysis reveals that isopeptide antibodies do not specifically recognize the epsilon-(gamma-glutamyl)lysine bonds formed by transglutaminase activity. J Neurosci Methods 134:151-8 (2004). PubMed: 15003381
- el Alaoui S et al. Transglutaminase activity and N epsilon (gamma glutamyl) lysine isopeptide levels during cell growth: an enzymic and immunological study. Int J Cancer 48:221-6 (1991). PubMed: 1673452
- Roch AM et al. Differential expression of isopeptide bonds N epsilon (gamma-glutamyl) lysine in benign and malignant human breast lesions: an immunohistochemical study. Int J Cancer 48:215-20 (1991). PubMed: 1673451
