Isocitrate Dehydrogenase Assay Kit (ab272530)

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Isocitrate Dehydrogenase Assay Kit Titration Curves

    Key features and details

    • Assay type: Quantitative
    • Detection method: Colorimetric
    • Sample type: Plasma, Serum, Tissue Culture Media

    製品の概要

    • 製品名

      Isocitrate Dehydrogenase Assay Kit
      Isocitrate Dehydrogenase キット 製品一覧

    • 検出方法

      Colorimetric

    • サンプルの種類

      Serum, Plasma, Tissue Culture Media

    • アッセイタイプ

      Quantitative

    • 検出範囲

      0.1 - 100

    • 製品の概要

      This kit (ab272530) measures the activity of the NADP+ isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. Our non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.


      Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 100 U/L for 30 min reaction.


      Convenient and high-throughput. Homogeneous "mix-incubatemeasure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

    • アプリケーション

      適用あり: Functional Studiesmore details

    製品の特性

    • 保存方法

      Store at -20°C. Please refer to protocols.
    • 内容 100 tests
      Assay Buffer 1 x 10ml
      Calibrator 1 x 1.5ml
      Diaphorase 1 x 120µl
      NADP/MTT 1 x 1ml
      Substrate 1 x 1ml

    • 研究分野

    • 関連性

      Isocitrate dehydrogenase (IDH; EC 1.1.1.41, NAD+) is an enzyme that participates in the citric acid cycle. These IDH3 isoforms catalyze the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate to oxalosuccinate, followed by the decarboxylation of the beta-carboxyl group to form the ketone, alpha-ketoglutarate. Other isoforms (EC 1.1.1.42, NADP+) catalyze the same reaction, but unrelated to the citric acid cycle. It is carried out in the mitochondrion (IDH2) as well as in the cytosol and peroxisome (IDH1) and use NADP+ as a cofactor instead of NAD+.

    • 細胞内局在

      Cytoplasm. Peroxisome

    • 別名

      • Cytosolic NADP isocitrate dehydrogenase
      • EC=1.1.1.42
      • IDH
      • IDP
      • Isocitrate dehydrogenase [NADP] cytoplasmic
      • NADP(+) specific ICDH
      • Oxalosuccinate decarboxylase
      • PICD
      see all

    アプリケーション

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab272530 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies
    Use at an assay dependent concentration.
    特記事項
    Functional Studies
    Use at an assay dependent concentration.

    画像

    • Isocitrate Dehydrogenase Assay Kit Titration Curves
      Isocitrate Dehydrogenase Assay Kit Titration Curves

      Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

      Example of Isocitrate Dehydrogenase titration curves.

      OD565 readings (left) and calculated activity (right).

    参考文献 (0)

    ab272530 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272530 は論文での使用が確認できていません。

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