Name: Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
Brand: RabMAb
SKU: ab109517
Rating: 5 (6 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
Reacts with: Mouse, Chicken, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品名

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
Islet 1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
由来種

Rabbit
特異性

86% identities with Islet 2
アプリケーション

適用あり: ICC/IF, WB, IP, Flow Cyt (Intra)more details
種交差性

交差種: Mouse, Chicken, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates. IHC-Fr: Chicken hindbrain tissue. ICC/IF: U-87 MG cell line.
特記事項
Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.

Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP4182
アイソタイプ

IgG
研究分野
- Neuroscience
- Development

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109517の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF	(1)	1/250. For unpurified use at 1/50 - 1/100.
WB		1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
IP		1/10 - 1/100.
Flow Cyt (Intra)		Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

特記事項
ICC/IF 1/250. For unpurified use at 1/50 - 1/100.
WB 1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
Flow Cyt (Intra) Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

機能

Binds to one of the cis-acting domain of the insulin gene enhancer.
組織特異性

Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
配列類似性

Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
細胞内局在

Nucleus.
Target information above from: UniProt accession P61371 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Omim: 600366 Human
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
- Unigene: 505 Human
- Unigene: 42242 Mouse
別名
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
- ISL 1 antibody
- ISL LIM homeobox 1 antibody
- ISL1 antibody
- ISL1 transcription factor LIM homeodomain antibody
- ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
- ISL1 transcription factor, LIM/homeodomain antibody
- ISL1_HUMAN antibody
- Islet-1 antibody
- Islet1 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

All lanes : Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (unpurified)

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 39 kDa
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.
Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 µg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Flow cytometry protocols
Immunoprecipitation protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab109517 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109517 は 40 報の論文で使用されています。

Hosoya M et al. The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development. FEBS J 288:325-353 (2021). PubMed: 32323465
Domínguez-Bautista JA et al. Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord. Front Cell Dev Biol 9:587096 (2021). PubMed: 33575260
Li Y et al. 4-phenylbutyrate exerts stage-specific effects on cardiac differentiation via HDAC inhibition. PLoS One 16:e0250267 (2021). PubMed: 33882103
Shi Q et al. Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancer. Mol Med 27:47 (2021). PubMed: 33962568
Huang X et al. Human amyotrophic lateral sclerosis excitability phenotype screen: Target discovery and validation. Cell Rep 35:109224 (2021). PubMed: 34107252

View all Publications for this product

レビューと Q&A

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1-6 of 6 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (anoderm)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer

Permeabilization

Specification

anoderm

Blocking step

anti-body diluent (BSA) as blocking agent for 30 minute(s) · Concentration: 00% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

投稿 May 17 2021

Application

IHC - Wholemount

Sample

Zebrafish Embryo (Brain)

Specification

Brain

Mr. Enrico Mingardo

Verified customer

投稿 Nov 22 2019

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Skin)

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C

Fixative

Paraformaldehyde

MR. Elena Ezhkova

Verified customer

投稿 Jan 22 2018

Application

ChIP

Sample

Mouse Cell lysate - nuclear (Min6)

Negative control

Negative control for Isl-1 antibody: known locus without enrichment Positive control for IP: Antibody for another factor that is not enriched at that locus

Specification

Min6

Detection step

Real-time PCR

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1.1% Formaldehyed

Positive control

Positive control for Isl-1 antibody: known locus of enrichment Positive control for IP: Antibody for another factor also enriched at that locus

Abcam user community

Verified customer

投稿 Mar 24 2015

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Human pluripotent stem cell derived cardiomyocyte)

Permeabilization

Yes - saponin

Specification

Human pluripotent stem cell derived cardiomyocyte

Blocking step

Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Oct 16 2014

Application

Immunohistochemistry (Frozen sections)

Sample

Chicken Tissue sections (hindbrain section)

Permeabilization

Yes - Triton-X

Specification

hindbrain section

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Oct 17 2013

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (40)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

IHC - Wholemount abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

Immunohistochemistry (Frozen sections) abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

ChIP abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

Immunohistochemistry (Frozen sections) abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker