Anti-Islet 1 抗体 [EP4182] - Neural Stem Cell Marker (ab109517)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
- Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Chicken, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
Islet 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker -
由来種
Rabbit -
特異性
86% identities with Islet 2 -
アプリケーション
適用あり: ICC/IF, WB, IP, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Chicken, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates. IHC-Fr: Chicken hindbrain tissue. ICC/IF: U-87 MG cell line.
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特記事項
Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.
Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP4182 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203404)
- Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203405)
- Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203406)
- Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
- Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] (ab275990)
- Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab277925)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109517の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
1/250.
For unpurified use at 1/50 - 1/100. |
WB |
1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
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IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/250. For unpurified use at 1/50 - 1/100. |
WB
1/10000 - 1/50000. Predicted molecular weight: 39 kDa. |
IP
1/10 - 1/100. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Binds to one of the cis-acting domain of the insulin gene enhancer. -
組織特異性
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. -
配列類似性
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Omim: 600366 Human
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
- Unigene: 505 Human
- Unigene: 42242 Mouse
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別名
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
画像
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Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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All lanes : Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (unpurified)
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.
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Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 µg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (52)
ab109517 は 52 報の論文で使用されています。
- Wang W et al. The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development. Nat Commun 13:958 (2022). PubMed: 35177643
- Akter M et al. Generation of highly pure motor neurons from human induced pluripotent stem cells. STAR Protoc 3:101223 (2022). PubMed: 35300000
- Hosoya M et al. Early development of the cochlea of the common marmoset, a non-human primate model. Neural Dev 17:6 (2022). PubMed: 35524278
- Bauer US et al. Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing. Front Integr Neurosci 16:855071 (2022). PubMed: 35669734
- Kishimoto K et al. Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems. Nat Protoc 17:2699-2719 (2022). PubMed: 35978039