Anti-IRSp53 抗体 - C-terminal (ab15697)
Key features and details
- Goat polyclonal to IRSp53 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Dog, Human
- Isotype: IgG
製品の概要
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製品名
Anti-IRSp53 antibody - C-terminal
IRSp53 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IRSp53 - C-terminal -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Dog, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human IRSp53 aa 512-520 (C terminal).
Sequence:C-SGSGTLVSTV
(Peptide available asab23256) -
ポジティブ･コントロール
- WB: Human brain lysate.
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特記事項
This antibody is expected to recognise the human isoform 1 only of this protein.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
This antibody is expected to recognise the human isoform 1 only of this protein. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15697の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. -
組織特異性
Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta. -
配列類似性
Contains 1 IMD (IRSp53/MIM homology) domain.
Contains 1 SH3 domain. -
ドメイン
The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH. -
翻訳後修飾
Phosphorylated on tyrosine residues by INSR in response to insulin treatment. -
細胞内局在
Cytoplasm. Membrane. Cell projection > filopodium. Cell projection > ruffle. Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles. - Information by UniProt
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参照データベース
- Entrez Gene: 403473 Dog
- Entrez Gene: 10458 Human
- Entrez Gene: 108100 Mouse
- Entrez Gene: 117542 Rat
- GenBank: NP_006331.1 Human
- Omim: 605475 Human
- SwissProt: Q9UQB8 Human
- SwissProt: Q8BKX1 Mouse
see all -
別名
- BAI-associated protein 2 antibody
- BAI1 associated protein 2 antibody
- BAI1-associated protein 2 antibody
see all
画像
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Western blot - Anti-IRSp53 antibody - C-terminal (ab15697)Anti-IRSp53 antibody - C-terminal (ab15697) at 1 µg/ml + Lysates prepared from human brain at 35 µg
Predicted band size: 60 kDaab15697 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab15697 は 3 報の論文で使用されています。
- Fedoryshchak RO et al. Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife 9:N/A (2020). PubMed: 32975518
- Goodwin JM et al. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Mol Cell 55:436-50 (2014). PubMed: 25042806
- Cohen D et al. The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell-ECM signaling. J Cell Biol 192:525-40 (2011). WB ; Dog . PubMed: 21282462
