Anti-IRS1 (phospho Y896) 抗体 (ab4873)

Submit a review Submit a question References (3)
Western blot - Anti-IRS1 (phospho Y896) antibody (ab4873)

    Key features and details

    • Rabbit polyclonal to IRS1 (phospho Y896)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-IRS1 (phospho Y896) antibody
      IRS1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to IRS1 (phospho Y896)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat

    • 免疫原

      Synthetic peptide derived from a region of IRS 1 that contains tyrosine 896. The sequence is conserved in human, mouse, and rat.

    • ポジティブ･コントロール

      • Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1 and stimulated with insulin.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab4873 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 165 kDa.

    ターゲット情報

    • 機能

      May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

    • 関連疾患

      Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

    • 配列類似性

      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • 翻訳後修飾

      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    画像

    • Western blot - Anti-IRS1 (phospho Y896) antibody (ab4873)
      Western blot - Anti-IRS1 (phospho Y896) antibody (ab4873)

      Peptide Competition:  Extracts prepared from CHO-T cells transiently transfected with human IRS-1 and treated with 50 nM insulin for 5 minutes were resolved by SDSPAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4873 antibody, following prior incubation with: no peptide (1), a generic phosphotyrosine containing peptide (2), the non-phosphopeptide corresponding to the immunogen (3), or the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4873 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

      Phosphatase Stripping: Extracts prepared from CHO-T cells transiently transfected with human IRS 1 and

    参考文献 (3)

    ab4873 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4873 は 3 報の論文で使用されています。

    • Tan J & Guo L Swimming intervention alleviates insulin resistance and chronic inflammation in metabolic syndrome. Exp Ther Med 17:57-62 (2019). PubMed: 30651765
    • Wang Y  et al. Silencing of SAA1 inhibits palmitate- or high-fat diet induced insulin resistance through suppression of the NF-?B pathway. Mol Med 25:17 (2019). PubMed: 31060494
    • Stuart CA  et al. Insulin resistance and muscle insulin receptor substrate-1 serine hyperphosphorylation. Physiol Rep 2:N/A (2014). PubMed: 25472611

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab4873.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com