Anti-IRS1 (phospho S312) 抗体 (ab4865)

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Western blot - Anti-IRS1 (phospho S312) antibody (ab4865)

    Key features and details

    • Rabbit polyclonal to IRS1 (phospho S312)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-IRS1 (phospho S312) antibody
      IRS1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to IRS1 (phospho S312)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Pig

    • 免疫原

      Synthetic peptide (Human) derived from a region of IRS 1 that contains serine 312.

    • ポジティブ･コントロール

      • Phorbol ester stimulated (TPA) Chinese Hamster Ovary cell line expressing human insulin receptor (CHO-T) and transiently transfected with a plasmid encoding human IRS 1.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab4865 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB 1/1000. Predicted molecular weight: 165 kDa.

    ターゲット情報

    • 機能

      May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

    • 関連疾患

      Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

    • 配列類似性

      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • 翻訳後修飾

      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    画像

    • Western blot - Anti-IRS1 (phospho S312) antibody (ab4865)
      Western blot - Anti-IRS1 (phospho S312) antibody (ab4865)
      Peptide Competition and Phosphatase Stripping: Extracts prepared from CHO-T cells transiently transfected with wild-type human IRS 1 and treated with TPA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were treated with lambda phosphatase (1) or left untreated (2-6), blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with 0.50 µg/mL ab4865 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1-3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4865 blocks the antibody signal, thereby demonstrating the specificity of the anti

    参考文献 (2)

    ab4865 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4865 は 2 報の論文で使用されています。

    • Martín-Segura A  et al. Age-associated cholesterol reduction triggers brain insulin resistance by facilitating ligand-independent receptor activation and pathway desensitization. Aging Cell 18:e12932 (2019). PubMed: 30884121
    • Dávila D & Torres-Aleman I Neuronal Death by Oxidative Stress Involves Activation of FOXO3 through a Two-Arm Pathway that Activates Stress Kinases and Attenuates Insulin-like Growth Factor I Signalling. Mol Biol Cell : (2008). WB ; Rat . PubMed: 18287535

    レビューと Q&A

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    Question

    Thank-you for the information on your products. I have a question regarding your two anti-phospho-IRS-1 S312 antibodies (ab3688 & ab4865). Are they two batches of the same thing and are they both available? Also I would like some clarification of the distinction between S312 and S307 as outlined in the datasheet for ab3688 (below). Am I correct in assuming that S312 is the human version of the rat S307, and that if I use this antibody in human tissue I will identify the S312 site and in rat tissue the S307?

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    Answer

    Ab3688 and Ab4865 are two different antibodies, we get them from two different sources. Both are in stock. Both have only been tested for cross-reactivity with human. Ab3688 is specific for human IRS 1 phosphorylated at the position of Serine 312. In humans Serine 312 is phosphorylated and in rats and mice Serine 307 is phosphorylated.

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