Anti-IRS1 (phospho S307) 抗体 (ab5599)

Submit a review Submit a question References (5)
Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)
  • Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)

Key features and details

  • Rabbit polyclonal to IRS1 (phospho S307)
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-IRS1 (phospho S307) antibody
    IRS1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to IRS1 (phospho S307)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICCmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, African green monkey

  • 免疫原

    Synthetic peptide corresponding to Human IRS1 aa 301-315.
    Sequence: ESITATSpPASMVGGK
    (Peptide available as ab41770)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysate; NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysates. ICC: T-47D cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5599の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ICC
Use a concentration of 1 - 2 µg/ml.
特記事項
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ICC
Use a concentration of 1 - 2 µg/ml.

ターゲット情報

  • 機能

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

  • 関連疾患

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

  • 配列類似性

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.

  • 翻訳後修飾

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

画像

  • Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)
    Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)

    Immunocytochemistry analysis of 4% paraformaldehyde-fixed  0.25% Triton™ X-100 permeabilized T-47D cells (Human ductal breast epithelial tumor cell line) staining IRS1 with ab5599 at 1 µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control uses no primary antibody. 

  • Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)
    Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)
    All lanes : Anti-IRS1 (phospho S307) antibody (ab5599) at 3 µg/ml

    Lane 1 : Jurkat whole cell lysates
    Lane 2 : Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysate
    Lane 3 : NIH/3T3 whole cell lysates
    Lane 4 : NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysates

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) secondary antibody, HRP conjugate at 0.4 µg/ml

    Developed using the ECL technique.

    Predicted band size: 131 kDa
    Observed band size: 131 kDa



    Blocking buffer: 5% skimmed milk

参考文献 (5)

ab5599 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5599 は 5 報の論文で使用されています。

  • Yun C  et al. Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway. Exp Ther Med 19:923-930 (2020). PubMed: 32010253
  • Velickovic N  et al. Modulation of hepatic inflammation and energy-sensing pathways in the rat liver by high-fructose diet and chronic stress. Eur J Nutr 58:1829-1845 (2019). PubMed: 29845385
  • Ma L  et al. Intermittent Hypoxia Composite Abnormal Glucose Metabolism-Mediated Atherosclerosis In Vitro and In Vivo: The Role of SREBP-1. Oxid Med Cell Longev 2019:4862760 (2019). PubMed: 30863480
  • Chang RC  et al. Programmed increases in LXRa induced by paternal alcohol use enhance offspring metabolic adaptation to high-fat diet induced obesity. Mol Metab 30:161-172 (2019). PubMed: 31767168
  • Garabadu D & Krishnamurthy S Diazepam potentiates the antidiabetic, antistress and anxiolytic activities of metformin in type-2 diabetes mellitus with cooccurring stress in experimental animals. Biomed Res Int 2014:693074 (2014). WB ; Rat . PubMed: 24995322

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab5599.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com