Anti-IRS1 (phospho S307) 抗体 (ab1194)

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Western blot - Anti-IRS1 (phospho S307) antibody (ab1194)

    Key features and details

    • Rabbit polyclonal to IRS1 (phospho S307)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-IRS1 (phospho S307) antibody
      IRS1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to IRS1 (phospho S307)

    • 由来種

      Rabbit

    • 特異性

      Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Pig

    • 免疫原

      Synthetic phospho peptide: GLTRRSRTESpITATSPASM conjugated to KLH, corresponding to amino acids 298-316 of Human IRS 1.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1194の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
    特記事項
    WB
    1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).

    ターゲット情報

    • 機能

      May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

    • 関連疾患

      Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

    • 配列類似性

      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • 翻訳後修飾

      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    画像

    • Western blot - Anti-IRS1 (phospho S307) antibody (ab1194)
      Western blot - Anti-IRS1 (phospho S307) antibody (ab1194)
      All lanes : Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min.

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 131 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?
      Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.



      The 180kDa band is believed to correspond to phospho S307 IRS1. Blocking with the immunising peptide removes the 180kDa band (data not shown). A 4-20% Tris-Glycine gel was used, with blocking with 5% goat serum and 0.5% BLOTTO in PBS.

    参考文献 (8)

    ab1194 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1194 は 8 報の論文で使用されています。

    • Tian H  et al. Effect of Dexmedetomidine-Mediated Insulin-Like Growth Factor 2 (IGF2) Signal Pathway on Immune Function and Invasion and Migration of Cancer Cells in Rats with Ovarian Cancer. Med Sci Monit 25:4655-4664 (2019). PubMed: 31230061
    • Liu SX  et al. Exercise Reduces Insulin Resistance in Type 2 Diabetes Mellitus via Mediating the lncRNA MALAT1/MicroRNA-382-3p/Resistin Axis. Mol Ther Nucleic Acids 18:34-44 (2019). PubMed: 31479923
    • Gallo JRB  et al. Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery. Plast Reconstr Surg Glob Open 7:e2339 (2019). PubMed: 31592376
    • Prabakaran AD  et al. Identification of early indicators of altered metabolism in normal development using a rodent model system. Dis Model Mech 11:N/A (2018). PubMed: 29434026
    • Jiang J  et al. Oncogenic activity of insulin in the development of non-small cell lung carcinoma. Oncol Lett 15:447-452 (2018). PubMed: 29387228
    • Vidal-Quadras M  et al. Endocytic turnover of Rab8 controls cell polarization. J Cell Sci 130:1147-1157 (2017). PubMed: 28137756
    • Ma SB  et al. Epigallocatechin-3-gallate ameliorates insulin resistance in hepatocytes. Mol Med Rep 15:3803-3809 (2017). PubMed: 28393248
    • Stuart CA  et al. Insulin resistance and muscle insulin receptor substrate-1 serine hyperphosphorylation. Physiol Rep 2:N/A (2014). PubMed: 25472611

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