Anti-IRS1 (phospho S307) 抗体 (ab1194)
製品の概要
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製品名Anti-IRS1 (phospho S307) antibody
IRS1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to IRS1 (phospho S307)
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由来種Rabbit
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特異性Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation.
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Pig
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免疫原
Synthetic phospho peptide: GLTRRSRTESpITATSPASM conjugated to KLH, corresponding to amino acids 298-316 of Human IRS 1.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa). |
ターゲット情報
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機能May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
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関連疾患Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
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配列類似性Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻訳後修飾Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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参照データベース
- Entrez Gene: 3667 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 25467 Rat
- Omim: 147545 Human
- SwissProt: P35568 Human
- SwissProt: P35569 Mouse
- SwissProt: P35570 Rat
- Unigene: 471508 Human
see all -
別名
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
画像
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Western blot - Anti-IRS1 (phospho S307) antibody (ab1194)All lanes : Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution
Lane 1 : 293 cell lysate
Lane 2 : 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min.
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
The 180kDa band is believed to correspond to phospho S307 IRS1. Blocking with the immunising peptide removes the 180kDa band (data not shown). A 4-20% Tris-Glycine gel was used, with blocking with 5% goat serum and 0.5% BLOTTO in PBS.
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Prabakaran AD et al. Identification of early indicators of altered metabolism in normal development using a rodent model system. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29434026) »
- Jiang J et al. Oncogenic activity of insulin in the development of non-small cell lung carcinoma. Oncol Lett 15:447-452 (2018). Read more (PubMed: 29387228) »
