Anti-IRS1+IRS2 抗体 [EP263Y] - BSA and Azide free (ab247286)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP263Y] to IRS1 + IRS2 - BSA and Azide free
- Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-IRS1+IRS2 antibody [EP263Y] - BSA and Azide free -
製品の詳細
Rabbit monoclonal [EP263Y] to IRS1 + IRS2 - BSA and Azide free -
由来種
Rabbit -
特異性
The immunogen used for this product shares 78% homology with IRS2. Cross-reactivity with this protein has not been confirmed experimentally.
-
アプリケーション
適用あり: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: HEK-293T transfected with a human IRS1 expression vector containing a Myc/DDK tag whole cell lysate, HEK-293T transfected with a human IRS2 expression vector containing a Myc/DDK tag whole cell lysate.
-
特記事項
ab247286 is the carrier-free version of ab40777.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP263Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247286の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 170 kDa (predicted molecular weight: 130 kDa).
|
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 170 kDa (predicted molecular weight: 130 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
細胞内局在
IRS2: Cytoplasm > cytosol. -
参照データベース
- Entrez Gene: 3667 Human
- Entrez Gene: 8660 Human
- Omim: 147545 Human
- Omim: 600797 Human
- SwissProt: P35568 Human
- SwissProt: Q9Y4H2 Human
- Unigene: 442344 Human
- Unigene: 471508 Human
画像
-
Lanes 1-3 : Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/1000 dilution
Lanes 4-6 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution
Lanes 1 & 4 : HEK-293T transfected with an empty vector whole cell lysate
Lanes 2 & 5 : HEK-293T transfected with a human IRS1 expression vector containing a Myc/DDK tag whole cell lysate
Lanes 3 & 6 : HEK-293T transfected with a human IRS2 expression vector containing a Myc/DDK tag whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?This data was developed using ab40777, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: Lane 1, 2, 4, 5 , 6: 1 second. Lane 3: 80 seconds.
-
Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/10000 dilution (purified) + HEK293 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?This data was developed using ab40777, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
This data was developed using ab40777, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling IRS1 with purified ab40777 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. -
This data was developed using ab40777, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling IRS1 with purified ab40777 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used. Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
-
Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) (purified) + HeLa whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?This data was developed using ab40777, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
This data was developed using ab40777, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling IRS1 with unpurified ab40777 at 1/90 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/1000 dilution (unpurified) + HeLa cell lysate at 10 µg
Predicted band size: 130 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?This data was developed using ab40777, the same antibody clone in a different buffer formulation.
-
This data was developed using ab40777, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labelling IRS1 with unpurified ab40777. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab247286 は論文での使用が確認できていません。