Anti-IRS1 抗体 (ab131487)
Key features and details
- Rabbit polyclonal to IRS1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IRS1 antibody
IRS1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRS1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide conjugated to KLH, surrounding internal sequence amino acids 634-638 (P-M-S-P-K) of Human IRS1 (NP_005535.1).
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ポジティブ･コントロール
- 293 cell extract; Human breast carcinoma tissue; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab131487 was purified by affinity chromatography using epitope specific peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131487の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
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| IHC-P | (1) |
1/50 - 1/100.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa). |
|
IHC-P
1/50 - 1/100. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
関連疾患
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
配列類似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻訳後修飾
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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参照データベース
- Entrez Gene: 3667 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 25467 Rat
- Omim: 147545 Human
- SwissProt: P35568 Human
- SwissProt: P35569 Mouse
- SwissProt: P35570 Rat
- Unigene: 471508 Human
see all -
別名
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
画像
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Western blot - Anti-IRS1 antibody (ab131487)Anti-IRS1 antibody (ab131487) at 1/500 dilution + 293 cell extract
Predicted band size: 131 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-IRS1 antibody (ab131487)Immunofluorescent analysis of methanol-fixed HeLa cells labelling IRS1 with ab131487 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 antibody (ab131487)Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling IRS1 with ab131487 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (11)
ab131487 は 11 報の論文で使用されています。
- Xu W et al. Anti-inflammatory activities of puerarin in high-fat diet-fed rats with streptozotocin-induced gestational diabetes mellitus. Mol Biol Rep 47:7537-7546 (2020). PubMed: 32946041
- Li L et al. Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice. Food Funct 11:8743-8756 (2020). PubMed: 32955050
- Li D et al. Gonadal white adipose tissue-derived exosomal MiR-222 promotes obesity-associated insulin resistance. Aging (Albany NY) 12:22719-22743 (2020). PubMed: 33197889
- Yan R et al. miR-183-5p promotes proliferation and migration in hepatocellular carcinoma by targeting IRS1 and its association with patient survival. Int J Biol Markers 35:83-89 (2020). PubMed: 32921226
- Zhang Y et al. Hyperinsulinemia Can Cause Kidney Disease in the IGT Stage of OLETF Rats via the INS/IRS-1/PI3-K/Akt Signaling Pathway. J Diabetes Res 2019:4709715 (2019). PubMed: 31737684
