IRGM 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to IRGM
アプリケーション適用あり: WBmore details
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IRGM.
- This antibody gave a positive signal in the following human tissue lysates: Small Intestine; Colon.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
精製度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab93901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 20 kDa).|
機能Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
組織特異性Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
関連疾患Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
配列類似性Belongs to the interferon-inducible GTPase family.
細胞内局在Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
- Information by UniProt
- IFI1 antibody
- Iigp3 antibody
- Iipg3 antibody
All lanes : Anti-IRGM antibody (ab93901) at 1 µg/ml
Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.