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Anti-IRF1 抗体 [BLR039F] - BSA free (ab272088)

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Immunoprecipitation - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
  • Immunocytochemistry - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
  • Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR039F] to IRF1 - BSA free
  • Suitable for: WB, ICC, IHC-P, IP
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-IRF1 antibody [BLR039F] - BSA free
    IRF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [BLR039F] to IRF1 - BSA free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide within Human IRF1 aa 250-300. The exact sequence is proprietary. NP_002189.1 and Gene ID 3659.
    Database link: P10914

  • 特記事項

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272088 is the carrier-free version of ab243895. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 8.20
    Preservative: 0.09% Sodium azide
    Constituent: 98% Borate buffered saline
  • Concentration information loading...

  • 特記事項（精製）

    Recombinant antibody was purified from cell culture supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    BLR039F

  • アイソタイプ

    IgG

  • 研究分野

関連製品

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC
Human
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor.

  • 関連疾患

    Defects in IRF1 are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.

  • 配列類似性

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

  • 翻訳後修飾

    Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.
    Ubiquitinated. Appears to compete with sumoylation on C-terminal sites.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Interferon regulatory factor 1 antibody
      • Interferon regulatory factor 1 isoform +I9 antibody
      • Interferon regulatory factor 1 isoform d78 antibody
      • Interferon regulatory factor 1 isoform delta4 antibody
      • Interferon regulatory factor 1 isoform delta7 antibody
      • IRF 1 antibody
      • IRF-1 antibody
      • IRF1 antibody
      • IRF1_HUMAN antibody
      • MAR antibody
      • MAR1 antibody
      see all

    画像

    • Immunoprecipitation - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
      Immunoprecipitation - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)

      IRF1 was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243895 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243895 at 1/1000 dilution.

      Lane 1: ab243895 IP in Jurkat whole cell lysate.

      Lane 2: Control IgG in Jurkat whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243895).

    • Immunocytochemistry - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
      Immunocytochemistry - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
      Formalin-fixed, paraffin-embedded OVCAR-8 cells labeling IRF1 using ab243895 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243895).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
      Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for IRF1 using ab243895 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243895).

    • Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)
      Anti-IRF1 antibody [BLR039F] - BSA free (ab272088)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272088 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272088 は論文での使用が確認できていません。

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