Application
Western blot
Sample
Human Cell lysate - whole cell (U937 cells)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycin gel)
Loading amount
40 µg
Specification
U937 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody #7076
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal™ West Femto Maximum Sensitiv
Exposure
12 minute(s) and 0 second(s)
Additional data
Additional Notes
I have tried this ab on wb with U937, DLD1, HT116 and MDA231 cells. None gave a signal
Abcam response
Thank you for your honest feedback. We are sorry to hear that the antibody did not work as expected. The antibody is tested in human samples and we resolved this issue in accordance with our AbPromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 08 2021