製品の概要
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製品名Anti-IRAK-1 antibody
IRAK-1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to IRAK-1
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由来種Rabbit
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特異性No cross reactivity to IRAK-2.
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アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Human, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Human IRAK-1 aa 700-712 (C terminal).
(Peptide available asab6230) -
ポジティブ･コントロール
- THP-1 or HeLa whole cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C.
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バッファーPreservative: 0.02% Sodium azide
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Concentration information loading... -
精製度Affinity purified
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特記事項（精製）Affinity chromatography purified via peptide column.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 80 kDa.Can be blocked with IRAK-1 peptide (ab6230). (THP-1 or HeLa whole cell) from non-activated cells. | |
| IP | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
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組織特異性Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
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配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. - Information by UniProt
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参照データベース
- Entrez Gene: 3654 Human
- Entrez Gene: 16179 Mouse
- Entrez Gene: 363520 Rat
- Omim: 300283 Human
- SwissProt: P51617 Human
- SwissProt: Q62406 Mouse
- Unigene: 522819 Human
- Unigene: 38241 Mouse
see all -
別名
- AA48924 antibody
- Il1rak antibody
- Interleukin 1 receptor associated kinase 1 antibody
see all
画像
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Western blot - Anti-IRAK-1 antibody (ab238)All lanes : Anti-IRAK-1 antibody (ab238) at 1/2000 dilution
Lane 1 : THP-1 (THP)whole cell lysate
Lane 2 : HeLa (HL) whole cell lysate
Predicted band size: 80 kDa
Observed band size: 80 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody (ab238)ab238 at 10µg/ml staining Hela cells by ICC/IF -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody (ab238)IHC image of ab238 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab238, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Flow Cytometry - Anti-IRAK-1 antibody (ab238)Image courtesy of an anonymous Abreview.ab238 staining IRAK-1 in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.P : Permeabilized US : Unstained (Red Peak) IGG RB : IgG Rabbit (Blue Peak) IRAK Ab (Green Peak)
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody (ab238)Image courtesy of Dr Mahesh Shivananjappa by Abreview.ab238 staining IRAK-1 in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab238 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.
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Immunoprecipitation - Anti-IRAK-1 antibody (ab238)IRAK was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to IRAK-1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab238.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 80kDa: IRAK-1 -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody (ab238)Immunofluorescence of IRAK-1 in HeLa cells using ab238 at 20 ug/ml.
プロトコール
参考文献
This product has been referenced in:
- Chen Y et al. MicroRNA-146a-5p attenuates irradiation-induced and LPS-induced hepatic stellate cell activation and hepatocyte apoptosis through inhibition of TLR4 pathway. Cell Death Dis 9:22 (2018). WB ; Mouse . Read more (PubMed: 29348414) »
- Wang Z et al. Chronic constriction injury-induced microRNA-146a-5p alleviates neuropathic pain through suppression of IRAK1/TRAF6 signaling pathway. J Neuroinflammation 15:179 (2018). WB, IHC (PFA fixed) ; Rat . Read more (PubMed: 29885668) »
レビューと Q&A
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