Anti-IRAK-1 抗体 (ab238)
Key features and details
- Rabbit polyclonal to IRAK-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Rhesus monkey
- Isotype: IgG
製品の概要
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製品名
Anti-IRAK-1 antibody
IRAK-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRAK-1 -
由来種
Rabbit -
特異性
At least four isoforms of IRAK are known to exist; this antibody will detect all four isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
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アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human IRAK-1 aa 700-712 (C terminal).
(Peptide available asab6230) -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Affinity chromatography purified via peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | 1/1000. Predicted molecular weight: 77 kDa. (THP-1 or HeLa whole cell) from non-activated cells. |
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IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. -
組織特異性
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. - Information by UniProt
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参照データベース
- Entrez Gene: 3654 Human
- Entrez Gene: 16179 Mouse
- Entrez Gene: 363520 Rat
- Omim: 300283 Human
- SwissProt: P51617 Human
- SwissProt: Q62406 Mouse
- Unigene: 522819 Human
- Unigene: 38241 Mouse
see all -
別名
- AA48924 antibody
- Il1rak antibody
- Interleukin 1 receptor associated kinase 1 antibody
see all
画像
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All lanes : Anti-IRAK-1 antibody (ab238) at 1 µg/ml
Lane 1 : L1210 cell lysate
Lane 2 : YB2/0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa1h incubation at RT in 5% NFDM/TBST.
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ab238 at 10µg/ml staining Hela cells by ICC/IF
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IHC image of ab238 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab238, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody (ab238)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
ab238 staining IRAK-1 in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab238 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.
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Immunofluorescence of IRAK-1 in HeLa cells using ab238 at 20 ug/ml.
プロトコール
データシートおよび資料
参考文献 (16)
ab238 は 16 報の論文で使用されています。
- Yang G & Zhao Y Overexpression of miR-146b-5p Ameliorates Neonatal Hypoxic Ischemic Encephalopathy by Inhibiting IRAK1/TRAF6/TAK1/NF-aB Signaling. Yonsei Med J 61:660-669 (2020). PubMed: 32734729
- Chu H et al. MicroRNA-206 promotes lipopolysaccharide-induced inflammation injury via regulation of IRAK1 in MRC-5 cells. Int Immunopharmacol 73:590-598 (2019). PubMed: 31279225
- Ji L et al. Jieduquyuziyin Prescription-Treated Rat Serum Suppresses Activation of Peritoneal Macrophages in MRL/Lpr Lupus Mice by Inhibiting IRAK1 Signaling Pathway. Evid Based Complement Alternat Med 2019:2357217 (2019). PubMed: 31781262
- Lindeløv Vestergaard A et al. MicroRNAs and histone deacetylase inhibition-mediated protection against inflammatory ß-cell damage. PLoS One 13:e0203713 (2018). PubMed: 30260972
- Gao Y et al. Overexpression of miR-146a blocks the effect of LPS on RANKL-induced osteoclast differentiation. Mol Med Rep 18:5481-5488 (2018). PubMed: 30387844