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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-IP3 receptor 抗体 [EPR4537] (ab108517)

Submit a review Q&A (1)References (4)
Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)
  • Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)
  • Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
  • OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517)
  • Anti-IP3 receptor antibody [EPR4537] (ab108517)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4537] to IP3 receptor
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-IP3 receptor antibody [EPR4537]
    IP3 receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4537] to IP3 receptor

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IPmore details
    適用なし: Flow Cyt or ICC

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat brain, SH-SY5Y, Mouse brain, and HeLa lysates IP: Mouse brain cells; IHC-P: Human and Mouse cerebellum tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108517の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 314 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 314 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
追加情報
Is unsuitable for Flow Cyt or ICC.

ターゲット情報

  • 機能

    Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.

  • 組織特異性

    Widely expressed.

  • 関連疾患

    Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.

  • 配列類似性

    Belongs to the InsP3 receptor family.
    Contains 5 MIR domains.

  • ドメイン

    The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.

  • 翻訳後修飾

    Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
    Phosphorylated on tyrosine residues.

  • 細胞内局在

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 4 antibody
    • 5-trisphosphate receptor antibody
    • 5-trisphosphate receptor type 1 antibody
    • DKFZp313E1334 antibody
    • DKFZp313N1434 antibody
    • inositol 1 4 5 triphosphate receptor type 1 antibody
    • Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
    • Inositol 1 antibody
    • InsP3R1 antibody
    • IP3 antibody
    • IP3 receptor antibody
    • IP3 receptor isoform 1 antibody
    • IP3R 1 antibody
    • IP3R antibody
    • IP3R1 antibody
    • ITPR 1 antibody
    • Itpr1 antibody
    • ITPR1_HUMAN antibody
    • SCA15 antibody
    • SCA16 antibody
    • SCA29 antibody
    • Type 1 inositol 1 4 5 trisphosphate receptor antibody
    • Type 1 inositol 1 antibody
    • Type 1 InsP3 receptor antibody
    see all

画像

  • Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
    Lane 3: Human brain tissue lysate (20 µg)
    Lane 4: SH-SY5Y cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.


    ab108517 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Immunohistochemical analysis of paraffin-embedded Human cerebellum  tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    ab108517 (purified) at 1:500 dilution (1.124 µg/ml) immunoprecipitating IP3 receptor in Mouse brain lysate. 
    Lane 1 (input): Mouse brain lysate 10µg
    Lane 2 (+): ab108517 & Mouse brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST.

  • Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    All lanes : Anti-IP3 receptor antibody [EPR4537] (ab108517) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : SH-SY5Y lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : HeLa brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 314 kDa

  • OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Anti-IP3 receptor antibody [EPR4537] (ab108517)

参考文献 (4)

ab108517 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108517 は 4 報の論文で使用されています。

  • Yoneda A  et al. Heat shock protein 47 confers chemoresistance on pancreatic cancer cells by interacting with calreticulin and IRE1a. Cancer Sci 112:2803-2820 (2021). PubMed: 34109710
  • Huang X  et al. Gli1+ Cells Residing in Bone Sutures Respond to Mechanical Force via IP3R to Mediate Osteogenesis. Stem Cells Int 2021:8138374 (2021). PubMed: 34434241
  • Bowie E & Goetz SC TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons. Elife 9:N/A (2020). PubMed: 31934864
  • da Rosa MS  et al. 3-Hydroxy-3-Methylglutaric Acid Impairs Redox and Energy Homeostasis, Mitochondrial Dynamics, and Endoplasmic Reticulum-Mitochondria Crosstalk in Rat Brain. Neurotox Res 37:314-325 (2020). PubMed: 31721046

レビューと Q&A

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レビューを送る 質問を送る

Question

antibody crossreaction to bovine ITPRs

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Answer

Please find here below the promised information about the ab108517 as well. I know it might not be useful to you anymore, but I received now the answer from the laboratory. As already mentioned, we do not have tested this antibody against bovine proteins. Please find here however the results of the alignments of the immunogen of ab108517 with the different bovine sequences: ITPR1 - 100% aas homology ITPR2 - ~90% aas homology ITPR3 - ~90% aas homology This information is surely good to keep in mind, if the other antibody you are currently testing will not crossreact to the bovine protein. I wish you good success with your research and am looking forward to hear back from you.

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