Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4536] to IP3 receptor - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Knockout validated
- Reacts with: Human
製品名Anti-IP3 receptor antibody [EPR4536] - BSA and Azide free
IP3 receptor 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4536] to IP3 receptor - BSA and Azide free
アプリケーション適用あり: IHC-P, Flow Cyt, WBmore details
非交差種: Mouse, Rat
Synthetic peptide within Human IP3 receptor aa 1-100. The exact sequence is proprietary.
Ab248112 is the carrier-free version of ab125076. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248112 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 320 kDa (predicted molecular weight: 314 kDa).|
機能Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.
関連疾患Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.
配列類似性Belongs to the InsP3 receptor family.
Contains 5 MIR domains.
ドメインThe receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
翻訳後修飾Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
Phosphorylated on tyrosine residues.
細胞内局在Endoplasmic reticulum membrane.
- Information by UniProt
- 4 antibody
- 5-trisphosphate receptor antibody
- 5-trisphosphate receptor type 1 antibody
All lanes : Anti-IP3 receptor antibody [EPR4536] (ab125076) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HuT78 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 314 kDa
This data was developed using ab125076, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.