Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS Tween 0.05%, BSA 1%, 2% goat serum
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Unable to get fluorescent signal
Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. In line with our Abpromise guarantee (https://www.abcam.com/abpromise), the customer received a replacement with Anti-Involucrin antibody [EPR13054](ab181980) which has worked well (https://www.abcam.com/involucrin-antibody-epr13054-ab181980/reviews/74152?productWallTab=Abreviews).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Laurène ROMANO
Verified customer
投稿 May 27 2020