製品の概要

  • 製品名
    Anti-Involucrin antibody [SY5]
    Involucrin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SY5] to Involucrin
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Dog, Human, Pig, Gorilla, Owl monkey
    非交差種: Mouse
  • 免疫原

    Pure involucrin from human keratinocytes.

  • エピトープ
    The epitope maps between codon 421-568 of human involucrin.
  • ポジティブ･コントロール
    • MCF-7 cells. Localized to upper spinous and granular layers in Normal skin.
  • 特記事項

    Involucrin is the differentiation marker of human keratinocytes.
    ab81216 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 - 0.2 µg/ml.
IHC-Fr 1/100.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • 組織特異性
    Keratinocytes of epidermis and other stratified squamous epithelia.
  • 配列類似性
    Belongs to the involucrin family.
  • 翻訳後修飾
    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • 細胞内局在
    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • 参照データベース
    • 別名
      • INVO_HUMAN antibody
      • Involucrin antibody
      • IVL antibody

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)

      Immunohistochemical analysis of Formalin fixed paraffin embedded human tonsil section labelling Involucrin with ab68 at dilution of 0.1 ug/mL. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)This image is courtesy of an anonymous abreview.
      ab68 at a 1/1000 dilution staining Involucrin in human normal skin tissue by Immunohistochemistry (formalin fixed, paraffin embedded sections), incubated for 2 hours at 21°C. Heat mediated antigen retrieval step performed using citrate buffer pH 6.0. Blocked with 10% serum for 30 minutes at 21°C. Secondary used at 1/100 polyclonal Donkey anti-mouse IgG conjugated to Alexa Fluor 594 (red).

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Involucrin antibody [SY5] (ab68)
      Immunohistochemistry (Frozen sections) - Anti-Involucrin antibody [SY5] (ab68)Image courtesy of anonymous abreviewer

      Immunohistochemical analysis of frozen Human artifical skin tissue labeling Involucrin with ab68 at 1/200 dilution.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody [SY5] (ab68)
      Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody [SY5] (ab68)

      ICC/IF image of ab68 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - Anti-Involucrin antibody [SY5] (ab68)
      Flow Cytometry - Anti-Involucrin antibody [SY5] (ab68)
      Overlay histogram showing A431 cells stained with ab68 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    参考文献

    This product has been referenced in:
    • Dziunycz PJ  et al. CYFIP1 is directly controlled by NOTCH1 and down-regulated in cutaneous squamous cell carcinoma. PLoS One 12:e0173000 (2017). WB ; Human . Read more (PubMed: 28410392) »
    • Davies EG  et al. Thymus transplantation for complete DiGeorge syndrome: European experience. J Allergy Clin Immunol 140:1660-1670.e16 (2017). IHC-P ; Human . Read more (PubMed: 28400115) »

    See all 24 Publications for this product

    ab68 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    14 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody [SY5]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Acetone
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Jul 14 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody [SY5]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (artificial skin)
    Permeabilization
    Yes - Triton X-100
    Specification
    artificial skin
    Blocking step
    Blocking One as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Aug 20 2015

    mouse tonsil

    1) Why are bands sen at 120 kDa and 170 kDa with human samples?
    2) Why is the antibody not good forouse? Data?
    3) send database links to human and mouse protein

    Thank you for your patience.
    I apologize for the delay, but I only heard back from the supplying lab today with the following information:

    "Wehave unfortunately no data to explain the differeing bands seen against human tissue.
    Read More

    precisions about the epitope, what is the aa sequence?
    possibility to have the full lenght protein?

    I am very sorry for the delayed response.

    Despite numerous reminders the source of the antibody only replied to me today with the following information:

    "We have no other information other than the codon sequence on the epitope f...

    Read More

    mouse tonsil

    1) Why are bands sen at 120 kDa and 170 kDa with human samples?
    2) Why is the antibody not good forouse? Data?
    3) send database links to human and mouse protein

    Thank you for contacting us.

    I apologize for the wait, but I have not yet heard back from the lab. I have sent another email to follow up with them.

    Thank you for your patience and understanding.

    mouse tonsil

    1) Why are bands seen at 120 kDa and 170 kDa with human samples?
    2) Why is the antibody not good for mouse? Data?
    3) send database links to human and mouse protein

    Thank you for contacting us.

    I have contacted the lab for the questions you had, and will let you know their answer.

    The database links for human and mouse proteins are as follows:

    human: http://www.uniprot.org/uniprot/P07...

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Involucrin antibody [SY5]

     Poor
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    abreview image
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Mar 19 2012

    Hello I am trying to gather MSDs’s all chemicals we keep for health and safety purposes, and One of them is : Involucrin mouse monoclonal cat no: ab68 Could you provise me with this document cause it is not accessible on the website. Thank you

    Thank you for contacting us.

    I have attached the MSDS for ab68.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody [SY5]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C
    Read More
    abreview image
    Username

    Dr. Vaughan Feisst

    Verified customer

    投稿 Oct 23 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Involucrin antibody [SY5]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal Skin)
    Specification
    Normal Skin
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrat, pH 6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Feb 19 2010

    1-10 of 14 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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