Anti-Involucrin 抗体 [SY5] (ab68)

Reviews (8)Q&A (7)References (27)

製品の概要

  • 製品名

    Anti-Involucrin antibody [SY5]
    Involucrin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [SY5] to Involucrin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, WB, IP, IHC-P, IHC-Fr, Flow Cytmore details

  • 種交差性

    交差種: Dog, Human, Pig, Gorilla, Owl monkey
    非交差種: Mouse

  • 免疫原

    Pure involucrin from human keratinocytes.

  • エピトープ

    The epitope maps between codon 421-568 of human involucrin.

  • ポジティブ･コントロール

    • IHC-P: Human tonsil, skin tissue. Localized to upper spinous and granular layers in normal skin. Flow cyt: A431 cells. ICC/IF: MCF7 cells.

  • 特記事項

    Involucrin is the differentiation marker of human keratinocytes.
    ab81216 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    SY5

  • ミエローマ

    P3x63

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab68 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 - 0.2 µg/ml.
IHC-Fr 1/100.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

  • 組織特異性

    Keratinocytes of epidermis and other stratified squamous epithelia.

  • 配列類似性

    Belongs to the involucrin family.

  • 翻訳後修飾

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

  • 細胞内局在

    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt

  • 参照データベース

    • 別名

      • INVO_HUMAN antibody
      • Involucrin antibody
      • IVL antibody

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)This image is courtesy of an anonymous abreview.
      ab68 at a 1/1000 dilution staining Involucrin in human normal skin tissue by Immunohistochemistry (formalin fixed, paraffin embedded sections), incubated for 2 hours at 21°C. Heat mediated antigen retrieval step performed using citrate buffer pH 6.0. Blocked with 10% serum for 30 minutes at 21°C. Secondary used at 1/100 polyclonal Donkey anti-mouse IgG conjugated to Alexa Fluor 594 (red).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)Chavez-Munoz, C. et al PLoS One. 2013 Dec 2;8(12):e80587. doi: 10.1371/journal.pone.0080587. eCollection 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      KLC (keratinocyte-like cells) have the capacity to stratify

      Human skin was decellularized and confirmed complete cell removal by H&E staining. ASC (human adipose-derived stem cells) were seeded on the epithelial side of human dermal decellularized matrices in the presence or absence of EGF (0.005 µg/mL). Human dermal decellularized matrices seeded with keratinocytes were used as controls. All three groups were kept in the same media conditions and lifted to an air-liquid interphase at the same time. Histological analysis revealed the presence of several layers of KLC with an organization similar to that of keratinocytes (A.1and 3). Surprisingly, ASC without EGF not only did not transdifferentiate into KLC, but instead migrated inside the matrix (A.2). Immunohistochemical analysis revealed a positive staining of cytokeratin-5 (B.3), cytokeratin-10 (C.3) and Involucrin (D.3) in stratified KLC matrices comparable to that of the stratified keratinocyte matrix (A, B, C and D.1). No positive staining was found in the ASC seeded matrices (A, B, C and D.2).

    • Flow Cytometry - Anti-Involucrin antibody [SY5] (ab68)
      Flow Cytometry - Anti-Involucrin antibody [SY5] (ab68)
      Overlay histogram showing A431 cells stained with ab68 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody [SY5] (ab68)
      Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody [SY5] (ab68)

      ICC/IF image of ab68 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY5] (ab68)

      Immunohistochemical analysis of Formalin fixed paraffin embedded human tonsil section labelling Involucrin with ab68 at dilution of 0.1 ug/mL. 

    • Immunohistochemistry (Frozen sections) - Anti-Involucrin antibody [SY5] (ab68)
      Immunohistochemistry (Frozen sections) - Anti-Involucrin antibody [SY5] (ab68)Image courtesy of anonymous abreviewer

      Immunohistochemical analysis of frozen Human artifical skin tissue labeling Involucrin with ab68 at 1/200 dilution.

      See Abreview

    参考文献

    This product has been referenced in:

    • Robinson DJ  et al. Epigenetic regulation of iASPP-p63 feedback loop in cutaneous squamous cell carcinoma. J Invest Dermatol N/A:N/A (2019). Read more (PubMed: 30710576) »
    • Kim YJ  et al. Topical administration of EGF suppresses immune response and protects skin barrier in DNCB-induced atopic dermatitis in NC/Nga mice. Sci Rep 8:11895 (2018). Read more (PubMed: 30093649) »
    See all 27 Publications for this product

    ab68 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-10 of 15 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Involucrin antibody [SY5]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (NHEKs (keratinocytes))
    Permeabilization
    No
    Specification
    NHEKs (keratinocytes)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 07 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody [SY5]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 14 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody [SY5]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (artificial skin)
    Permeabilization
    Yes - Triton X-100
    Specification
    artificial skin
    Blocking step
    Blocking One as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 20 2015

    Question

    mouse tonsil

    1) Why are bands sen at 120 kDa and 170 kDa with human samples?
    2) Why is the antibody not good forouse? Data?
    3) send database links to human and mouse protein

    Read More
    Answer

    Thank you for your patience.
    I apologize for the delay, but I only heard back from the supplying lab today with the following information:

    "Wehave unfortunately no data to explain the differeing bands seen against human tissue.

    The antibody was tested against mouse but showed no reactivity."

    I hope this information is nevertheless helpful to you.
    If you are not satisfied with the antibody, I'd be happy to replace, credit or refund it - if it was used in a tested species or application.Please do let me know.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    precisions about the epitope, what is the aa sequence?
    possibility to have the full lenght protein?

    Read More
    Answer

    I am very sorry for the delayed response.

    Despite numerous reminders the source of the antibody only replied to me today with the following information:

    "We have no other information other than the codon sequence on the epitope for this antibody.
    We are not able to supply the protein but should that ever be possible we will get in touch."

    I am sorry I could not be more helpful on this occasion. Please do not hesitate to contact me again if you have further questions.

    Read More

    Question

    mouse tonsil

    1) Why are bands sen at 120 kDa and 170 kDa with human samples?
    2) Why is the antibody not good forouse? Data?
    3) send database links to human and mouse protein

    Read More
    Answer

    Thank you for contacting us.

    I apologize for the wait, but I have not yet heard back from the lab. I have sent another email to follow up with them.

    Thank you for your patience and understanding.

    Read More

    Question

    mouse tonsil

    1) Why are bands seen at 120 kDa and 170 kDa with human samples?
    2) Why is the antibody not good for mouse? Data?
    3) send database links to human and mouse protein

    Read More
    Answer

    Thank you for contacting us.

    I have contacted the lab for the questions you had, and will let you know their answer.

    The database links for human and mouse proteins are as follows:

    human: http://www.uniprot.org/uniprot/P07476
    mouse: http://www.uniprot.org/uniprot/P48997

    I hope this information is of some help to you.
    In the meantime, please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Involucrin antibody [SY5]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    Mr. Carl Hobbs

    Verified customer

    投稿 Mar 19 2012

    Question

    Hello I am trying to gather MSDs’s all chemicals we keep for health and safety purposes, and One of them is : Involucrin mouse monoclonal cat no: ab68 Could you provise me with this document cause it is not accessible on the website. Thank you

    Read More
    Answer

    Thank you for contacting us.

    I have attached the MSDS for ab68.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-Involucrin antibody [SY5]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C
    Read More

    Dr. Vaughan Feisst

    Verified customer

    投稿 Oct 23 2010

    1-10 of 15 Abreviews or Q&A

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