Anti-Involucrin 抗体 [EPR13054] (ab181980)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13054] to Involucrin
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Involucrin antibody [EPR13054]
Involucrin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13054] to Involucrin -
由来種
Rabbit -
特異性
Unsuitable for rat IHC-P.
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アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- SW480 and Human fetal skin lysates; Human skin and tonsil tissues; HUVEC cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13054 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181980の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 68 kDa).
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特記事項 |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 68 kDa). |
ターゲット情報
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機能
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
組織特異性
Keratinocytes of epidermis and other stratified squamous epithelia. -
配列類似性
Belongs to the involucrin family. -
翻訳後修飾
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
細胞内局在
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
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参照データベース
- Entrez Gene: 3713 Human
- Entrez Gene: 60583 Rat
- Omim: 147360 Human
- SwissProt: P07476 Human
- SwissProt: P48998 Rat
- Unigene: 516439 Human
- Unigene: 142591 Rat
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別名
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
画像
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Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-Involucrin antibody [EPR13054] (ab181980) at 1/500000 dilution
Lane 1 : HaCaT (Human keratinocyte cell line) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteDiluting and blocking buffer: 5% NFDM/TBST
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All lanes :
Lane 1 : Human fetal skin tissue
Lane 2 : Rat skin tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteDiluting and blocking buffer: 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Involucrin with ab181980 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab181980 は 2 報の論文で使用されています。
- Miles RR et al. The eIF2 kinase GCN2 directs keratinocyte collective cell migration during wound healing via coordination of reactive oxygen species and amino acids. J Biol Chem 297:101257 (2021). PubMed: 34597669
- Zhou J et al. Transcriptomic Analysis of Healthy and Atopic Dermatitis Samples Reveals the Role of IL-37 in Human Skin. Immunohorizons 5:830-843 (2021). PubMed: 34702760