Anti-Interferon beta 抗体 (ab85803)
Key features and details
- Rabbit polyclonal to Interferon beta
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Interferon beta antibody
Interferon beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Interferon beta -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Macaque monkey -
免疫原
Synthetic peptide corresponding to Human Interferon beta aa 1-100 conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- Recombinant human Interferon beta protein (ab71475) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 and WI38 whole cell lysates and in the following human tissue lysates: Liver; Lung; Kidney; Spleen.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85803の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Has antiviral, antibacterial and anticancer activities. -
配列類似性
Belongs to the alpha/beta interferon family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3456 Human
- Entrez Gene: 15977 Mouse
- Omim: 147640 Human
- SwissProt: P01574 Human
- SwissProt: P01575 Mouse
- Unigene: 93177 Human
- Unigene: 1245 Mouse
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別名
- beta-interferon antibody
- Fibroblast interferon antibody
- IFB antibody
see all
画像
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All lanes : Anti-Interferon beta antibody (ab85803) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :WI-38 whole cell lysate (ab3960)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 120 kDa, 160 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The Interferon Beta protein sequence contains both a phosphorylation and a glycosylation site (Swissprot). Post-translation modifications could account for the migration of the band at a higher molecular weight than predicted. -
ICC/IF image of ab85803 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab85803 は 5 報の論文で使用されています。
- Abdelmoaty M et al. Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions. bioRxiv N/A:N/A (2021). PubMed: 34268510
- Abdelmoaty MM et al. Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions. Front Immunol 12:741502 (2021). PubMed: 34671355
- Li W et al. Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone. Theranostics 10:9032-9049 (2020). PubMed: 32802177
- Choi MR et al. Chloroquine Treatment Suppresses Mucosal Inflammation in a Mouse Model of Eosinophilic Chronic Rhinosinusitis. Allergy Asthma Immunol Res 12:994-1011 (2020). PubMed: 32935491
- Oishi I et al. Efficient production of human interferon beta in the white of eggs from ovalbumin gene-targeted hens. Sci Rep 8:10203 (2018). PubMed: 29976933