Anti-Interferon alpha 2b 抗体 [4E10] (ab9388)
Key features and details
- Mouse monoclonal [4E10] to Interferon alpha 2b
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-Interferon alpha 2b antibody [4E10]
Interferon alpha 2b 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4E10] to Interferon alpha 2b -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human Interferon alpha 2b.
Database link: P01563 -
ポジティブ・コントロール
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特記事項
The neutralising bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the test antibodies for 2 h at RT and then added to the cell monolayer. 24 h later the cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. Polyclonal antibodies to respective interferons and irrelevant monoclonal antibody of the same isotype were used as controls.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR317571-2, GR317571-3 and GR190761-4 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
4E10 -
ミエローマ
NS0/1 -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9388の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500.
|
特記事項 |
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WB
1/500. |
ターゲット情報
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機能
Produced by macrophages, IFN-alpha have antiviral activities. -
配列類似性
Belongs to the alpha/beta interferon family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3440 Human
- Omim: 147562 Human
- SwissProt: P01563 Human
- Unigene: 211575 Human
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別名
- Alpha 2a interferon antibody
- IFN alpha 2b antibody
- IFN-alpha-2 antibody
see all
画像
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Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4
µ g/ml).
Lane 1 - interferon alpha 2b
Lane 2 - prestained standard proteins.Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4
µg/ml).
Lane 1 - interferon alpha 2b
Lane 2 - prestained standard proteins.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab9388 は論文での使用が確認できていません。