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  1. Link

    interferon-alpha-2b-antibody-4e10-ab9388.pdf

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Immunology Innate Immunity Cytokines Interferons
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Anti-Interferon alpha 2b 抗体 [4E10] (ab9388)

  • Datasheet
  • SDS
Submit a review Q&A (6)

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Abpromise

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Western blot - Anti-Interferon alpha 2b antibody [4E10] (ab9388)

    Key features and details

    • Mouse monoclonal [4E10] to Interferon alpha 2b
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    HRP

    こちらの製品もご検討ください

    一次抗体
    Product image
    Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
    タンパク質
    Product image
    Recombinant Human Interferon alpha 2b protein (ab51094)
    一次抗体
    HRP Anti-Interferon alpha 2b antibody [4E10] (ab5258)

    関連製品

    製品の概要

    • 製品名

      Anti-Interferon alpha 2b antibody [4E10]
      Interferon alpha 2b 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [4E10] to Interferon alpha 2b
    • 由来種

      Mouse
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Recombinant full length protein corresponding to Human Interferon alpha 2b.
      Database link: P01563

    • ポジティブ・コントロール

      • ウェスタン・ブロット用ポジティブコントロールRecombinant Human Interferon alpha 2b protein
    • 特記事項

      The neutralising bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the test antibodies for 2 h at RT and then added to the cell monolayer. 24 h later the cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. Polyclonal antibodies to respective interferons and irrelevant monoclonal antibody of the same isotype were used as controls.

      This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR317571-2, GR317571-3 and GR190761-4 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7.2
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Protein A purified
    • ポリ/モノ

      モノクローナル
    • クローン名

      4E10
    • ミエローマ

      NS0/1
    • アイソタイプ

      IgG1
    • 軽鎖の種類

      unknown
    • 研究分野

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interferons
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interferons

    関連製品

    • Alternative Versions

      • HRP Anti-Interferon alpha 2b antibody [4E10] (ab5258)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human Interferon alpha 2b protein (ab51094)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9388の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500.
    特記事項
    WB
    1/500.

    ターゲット情報

    • 機能

      Produced by macrophages, IFN-alpha have antiviral activities.
    • 配列類似性

      Belongs to the alpha/beta interferon family.
    • 細胞内局在

      Secreted.
    • Target information above from: UniProt accession P01563 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 3440 Human
      • Omim: 147562 Human
      • SwissProt: P01563 Human
      • Unigene: 211575 Human
      • 別名

        • Alpha 2a interferon antibody
        • IFN alpha 2b antibody
        • IFN-alpha-2 antibody
        • IFNA antibody
        • Ifna2 antibody
        • IFNA2_HUMAN antibody
        • INFA2 antibody
        • Interferon alpha 2 antibody
        • Interferon alpha A antibody
        • Interferon alpha-2 antibody
        • Interferon alpha-A antibody
        • LeIF A antibody
        • LeIFA antibody
        see all

      画像

      • Western blot - Anti-Interferon alpha 2b antibody [4E10] (ab9388)
        Western blot - Anti-Interferon alpha 2b antibody [4E10] (ab9388)

        Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4 µg/ml).
        Lane 1 - interferon alpha 2b
        Lane 2 - prestained standard proteins.

        Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4 µg/ml).
        Lane 1 - interferon alpha 2b
        Lane 2 - prestained standard proteins.

      プロトコール

      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (0)

      ab9388 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab9388 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-6 of 6 Abreviews or Q&A

      Question

      Hello,



      I have recently ordered 2 vials of ab14039 (anti-interferon alpha 2b). I received both vials but one vial has a lot # GR76309-5 while the other vial has a lot# GR76309-4. Are these the same or different lot number? How much of these lot numbers do you have in stock? I am in the process of developing an assay that will require have a consistent lot # throughout method development, validation, and sample analysis.



      I have also purchased ab9388. My previous purchase had a lot # GR113176 and the most recent one has a lot # GR113176-1. Are these the same lot numbers?

      Read More

      Abcam community

      Verified customer

      Asked on May 09 2013

      Answer

      Thank you for contacting us. Yes, these are the same lot numbers in all cases. The number after the - just indicates the time it was aliquotted, so if the GRXXXX is the same but the dash is different you can be assured the lot is still the same. Please let me know if you have any further questions.

      Read More

      Abcam Scientific Support

      Answered on May 09 2013

      Question

      I have a question about one of your products,ab9388. I have used this antibody for concentration analysis of Interferon. I have analyzed both native and denatured Interferon and have found that the results between the two types of Interferon differ. Do you know if the antibody binds in a different way when the protein is denatured compared to when it is folded

      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Feb 14 2013

      Answer

      Thank you for contacting us.

      We have used this antibody under reducing and denaturing conditions in western blot. We unfortunately haven't done any comparison studies between native vs reducing conditions. I am sorry we are unable to comment on this due to lack of tested data.

      I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Feb 14 2013

      Question

      We would be interested in knowing how do you quantify your antibodies, especially for cat. no ab9386 and ab9388. Thanks in advance!

      Read More

      Abcam community

      Verified customer

      Asked on Jan 12 2012

      Answer

      Thank you for contacting us. Concentrations of the antibodies ab9386 and ab9388 are determined spectrophotometrically by an adsorbance measurement at 280 nm. The absorbance value (A280) is divided by the extiction coefficient of mouse IgG - 1.35. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Jan 12 2012

      Question

      I would like to know the quantity that will be provided in each tube lot after lot? I understand it’s 100 ul but would it be 200ug for each lot? Thanks in advance

      Read More

      Abcam community

      Verified customer

      Asked on Jan 11 2012

      Answer

      Thank you for contacting Abcam. For both ab9386 and ab9388, we currently sell them in 100ul aliquots, at a concentration of 2mg/ml. From what I can see, there are no plans to change this concentration for future lots of the antibody and since it is a mouse monoclonal there should not be a large variation in batch to batch concentrations. If any changes do occur then the new concentration will be clearly displayed on the datasheet and/or vial. Please let me know if there is anything else I can help you with.

      Read More

      Abcam Scientific Support

      Answered on Jan 11 2012

      Question

      I am trying to find an antibody to human interferon alpha that is biotinylated - do you sell such a product.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 20 2003

      Answer

      Thank you for your enquiry. We currently do not have an antibody to human interferon alpha that is biotinylated. We have the following human interferon alpha antibodies: ab9660, ab9386, ab8317, ab9388, and ab5258 (HRP conjugated). If you have any more questions, please contact us again.

      Read More

      Abcam Scientific Support

      Answered on Nov 20 2003

      Question

      Re. Mouse monoclonal (4E10) to Interferon alpha 2b & Mouse monoclonal (9D3) to human interferon alpha 2b I would like to use these two anitbodies together in ELISA. Can you send me a protocol please?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 03 2003

      Answer

      To use monoclonal antibodies 9D3 and 4E10 in sandwich ELISA for the detection of huIFN-alpha-2b, 4E10 antibody should be labeled either with horse-radish peroxidase or biotin. Monoclonal antibody 9D3 should be used as a capture antibody (for plate coating). Before labeling procedure, antibody 4E10 should be dialysed to remove sodium azide (see Abcam protocol Azide Removal by Dialysis). Biotinylation protocols are available on-line (see, for example: www.protocol-online.org or www.drmr.com). Biotinylated 4E10 can be detected using Streptavidin-Peroxidase (widely used reagent, see, for example www.chemicom.com or www.sigmaaldrich.com). Labeling of monoclonal antibody with peroxidase is more complicated procedure than biotinylation (see, for example, HRP Conjugation Kit description at www.4adi.com). After preparation of 4E10 conjugate, it can be used together with 9D3 in sandwich ELISA for the detection of huIFN-alpha-2b. Short protocol is given below: 1. Plate coating: 9D3, 5 mcg/ml in Na-carbonate buffer (pH 9.5), 100 mcl/well, incubation overnight at +4 C; 2. Plate blocking: 2% BSA in PBS, 100 mcl/well, 1 h at RT; 3. Washing: 3 times with PBST (PBS with 0.1% Tween-20); 4. Incubation with IFN standard and test samples: 1 h at RT, 100 mcl/well, dilution buffer – PBST; 5. Washing: 5-6 times with PBST; 6. Incubation with 4E10 conjugate: 1 h at RT, dilution buffer – PBST. If 4E10-biotin is used, further incubation step with Streptavidin-Peroxidase is required; 7. Washing: 8-10 times with PBST; 8. Development of enzymatic reaction using TMB-substrate.

      Read More

      Abcam Scientific Support

      Answered on Oct 07 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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