Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3t3 Fibroblasts)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
40 µg
Specification
NIH3t3 Fibroblasts
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA
Dilution
1/1000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: Rabbit IgG IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odyssey
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 130 kDa Non-specific: >250 kDa
Positive control
HEK cell extract
Additional data
Additional Notes
It works in Human cell extact but not a strong signal in mouse cell extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 21 2018