Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
N/A
Exposure
15 second(s)
Bands
Specific: ~130 kDa
Positive control
Non-treated cell
line1 shGFP
line2 shMT1-MMP
line 3 shGFP+Integrin beta1 overexpression
line 4 shMT1-MMP+Integrin beta1 overexpression
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 28 2019