Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human breast cancer metastasis)
Specification
Human breast cancer metastasis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TNB
Secondary antibody
Name
Non-Abcam antibody was used: Vector Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/500
Additional data
Additional Notes
This antibody would not work for me in a simple two-step fluorescent protocol. Appears rather sensitive to overfixation. It works well, however, with tyramide signal amplification or standard DAB techniques. Here, I've used the Perkin Elmer TSA-Cy3 kit. The image demonstrates a cancer metastasis at 40x with beta1 staining (in red) in both blood vessels and tumour cells. Blue is Hoechst for nuclei.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 22 2008