製品名Anti-Integrin alpha V beta 3 antibody [BV3]
Integrin alpha V beta 3 一次抗体 製品一覧
製品の詳細Mouse monoclonal [BV3] to Integrin alpha V beta 3
特異性This monoclonal antibody binds to human CD51 and Cd61 integrins present on human endothelium cells.
アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, IP, Flow Cytmore details
種交差性交差種: Rat, Chicken, Human
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.1% Sodium azide
Constituent: 0.2% BSA
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab7166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
関連性Function: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C (PubMed:20615244). Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for coxsackievirus A9 and B1 (PubMed:9426447, PubMed:15194773, PubMed:7519807). Integrin ITGAV:ITGB3 acts as a receptor for herpes virus 8/HHV-8 (PubMed:18045938). Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1 (PubMed:24367260). Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1 (PubMed:11160695). Integrin ITGAV:ITGB3 acts as a receptor for West nile virus (PubMed:23658209). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
細胞内局在Cell Membrane. Cytoplasmic. Integrin complex. Focal adhesion.
- Antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD 61 antibody
ab7166 staining human breast cancer cells by ICC/IF. Cells were paraformaldehyde fixed for 10 minutes and blocked in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours. An Alexa Fluor® 488 conjugated goat polyclonal to mouse antibody, diluted 1/200 was used as the secondary.
ab7166 staining Integrin alpha V beta 3 in human MeWo melanoma cells by Flow Cytometery. Cells were cultured in 24 well plates and harvested with 100 µL trypsin/ EDTA. Cells were fixed in 1:1 mixture of FACS/Flow and 4% paraformaldehyde in PBS. The primary antibody was diluted to 1 µg/ml and incubated with sample in 3% bovine serum albumin in phosphate buffer for 30 minutes at 37°C. An ab6785, FITC-conjugated goat polyclonal to mouse IgG (1/100) was used as secondary. A459 cells were used as negative control and showed no significant binding of the antibody. Different concentrations were evaluated and 1 µg/ml yield gave the best results. Blank cells were not treated with any antibodies at all, sec only cells were treated with secondary but not primary antibody.
ab7166 は 18 報の論文で使用されています。
- Faralli JA et al. Effect of avß3 Integrin Expression and Activity on Intraocular Pressure. Invest Ophthalmol Vis Sci 60:1776-1788 (2019). PubMed: 31022732
- He F et al. Reversal of EGFR inhibitors' resistance by co-delivering EGFR and integrin avß3 inhibitors with nanoparticles in non-small cell lung cancer. Biosci Rep 39:N/A (2019). PubMed: 31316001
- Bidone TC et al. Multiscale model of integrin adhesion assembly. PLoS Comput Biol 15:e1007077 (2019). PubMed: 31163027
- Perrucci GL et al. Integrin a?ß5 in vitro inhibition limits pro-fibrotic response in cardiac fibroblasts of spontaneously hypertensive rats. J Transl Med 16:352 (2018). PubMed: 30541573
- Zhao G et al. Clinical diagnosis of adult patients with acute megakaryocytic leukemia. Oncol Lett 16:6988-6997 (2018). PubMed: 30546432