製品の概要

  • 製品名

    Anti-Integrin alpha V beta 3 antibody [BV3]
    Integrin alpha V beta 3 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [BV3] to Integrin alpha V beta 3
  • 由来種

    Mouse
  • 特異性

    This monoclonal antibody binds to human CD51 and Cd61 integrins present on human endothelium cells.
  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, IP, Flow Cytmore details
    適用なし: WB
  • 種交差性

    交差種: Rat, Chicken, Human

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 関連性

      Function: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C (PubMed:20615244). Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for coxsackievirus A9 and B1 (PubMed:9426447, PubMed:15194773, PubMed:7519807). Integrin ITGAV:ITGB3 acts as a receptor for herpes virus 8/HHV-8 (PubMed:18045938). Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1 (PubMed:24367260). Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1 (PubMed:11160695). Integrin ITGAV:ITGB3 acts as a receptor for West nile virus (PubMed:23658209). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
    • 細胞内局在

      Cell Membrane. Cytoplasmic. Integrin complex. Focal adhesion.
    • 参照データベース

    • 別名

      • Antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD 61 antibody
      • CD51 antibody
      • CD51 antigen antibody
      • CD61 antibody
      • CD61 antigen antibody
      • DKFZp686A08142 antibody
      • GP3A antibody
      • GPIIIa antibody
      • Integrin alpha V antibody
      • Integrin alpha V vitronectin receptor alpha polypeptide antibody
      • Integrin alpha V vitronectin receptor alpha polypeptide antigen CD51 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • Integrin beta 3 antibody
      • Integrin beta 3 platelet glycoprotein IIIa antigen CD61 antibody
      • Integrin beta chain beta 3 antibody
      • integrinVbeta3 antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • MSK 8 antibody
      • MSK8 antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • Vitronectin receptor alpha polypeptide antibody
      • vitronectin receptor antibody
      • Vitronectin receptor subunit alpha antibody
      • VNR 1 antibody
      • VNR1 antibody
      • VNRA antibody
      see all

    画像

    • ab7166 staining human breast cancer cells by ICC/IF. Cells were paraformaldehyde fixed for 10 minutes and blocked in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours. An Alexa Fluor® 488 conjugated goat polyclonal to mouse antibody, diluted 1/200 was used as the secondary.

      See Abreview

    • ab7166 staining Integrin alpha V beta 3 in human MeWo melanoma cells by Flow Cytometery. Cells were cultured in 24 well plates and harvested with 100 µL trypsin/ EDTA. Cells were fixed in 1:1 mixture of FACS/Flow and 4% paraformaldehyde in PBS. The primary antibody was diluted to 1 µg/ml and incubated with sample in 3% bovine serum albumin in phosphate buffer for 30 minutes at 37°C. An ab6785, FITC-conjugated goat polyclonal to mouse IgG (1/100) was used as secondary. A459 cells were used as negative control and showed no significant binding of the antibody. Different concentrations were evaluated and 1 µg/ml yield gave the best results. Blank cells were not treated with any antibodies at all, sec only cells were treated with secondary but not primary antibody.

      See Abreview

    参考文献

    This product has been referenced in:

    • Faralli JA  et al. Effect of avß3 Integrin Expression and Activity on Intraocular Pressure. Invest Ophthalmol Vis Sci 60:1776-1788 (2019). Read more (PubMed: 31022732) »
    • Perrucci GL  et al. Integrin a?ß5 in vitro inhibition limits pro-fibrotic response in cardiac fibroblasts of spontaneously hypertensive rats. J Transl Med 16:352 (2018). Read more (PubMed: 30541573) »
    See all 15 Publications for this product

    レビューと Q&A

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    1-10 of 10 Abreviews

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate (10mM, pH 6.0)
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 23°C
    Fixative
    Paraformaldehyde

    Ms. Noureen Ali

    Verified customer

    投稿 Mar 26 2019

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Uterus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Cell Conditioning 1&2 (Roche Diagnostics)
    Permeabilization
    No
    Specification
    Uterus
    Blocking step
    Diluant Anticorps (Roche Diagnostics) as blocking agent for 32 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Formaldehyde

    Florian Chocteau

    Verified customer

    投稿 Nov 29 2017

    This product is known to not work in this application or species.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer cell line stably expressing Integrin)
    Loading amount
    60 µg
    Specification
    breast cancer cell line stably expressing Integrin
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

    Abcam user community

    Verified customer

    投稿 Jan 30 2012

    Application
    Flow Cytometry
    Sample
    Human Cell (MeWo melanoma cells)
    Specification
    MeWo melanoma cells
    Preparation
    Cell harvesting/tissue preparation method: Cells were cultured in 24 well plates and harvested with 100 µL trypsin/ EDTA
    Sample buffer: 3% bovine serum albumin in phosphate buffered saline (PBS/BSA)
    Fixation
    1:1 mixture of FACSFlow and 4% paraformaldehyde in PBS
    Permeabilization
    No
    Gating Strategy
    10,000 viable cells were evaluated in each experiment

    Prof. Olivia Merkel

    Verified customer

    投稿 Jul 14 2009

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (cervical)
    Specification
    cervical
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: tris-EDTA buffer
    Permeabilization
    No

    Mrs. Jinsuk An

    Verified customer

    投稿 May 20 2009

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Breast Cancer Cells)
    Specification
    Breast Cancer Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Dr. Sharon Sneddon

    Verified customer

    投稿 Oct 16 2008

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    投稿 May 08 2008

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 10mM citric acid buffer heated 10mins
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C

    Abcam user community

    Verified customer

    投稿 Apr 29 2008

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Chicken Tissue sections (ovary)
    Specification
    ovary
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citric acid
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

    Abcam user community

    Verified customer

    投稿 Jun 05 2007

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

    Abcam user community

    Verified customer

    投稿 Feb 07 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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