Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human skin SCC)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Human skin SCC
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/300
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Yong Sun Lee
Verified customer
投稿 Jun 27 2018