製品の概要

  • 製品名
    Anti-Integrin alpha V antibody [EPR16800]
    Integrin alpha V 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR16800] to Integrin alpha V
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment within Human Integrin alpha V aa 1-250. The exact sequence is proprietary.
    Database link: P06756

  • ポジティブ・コントロール
    • WB: HUVEC, HT-29, A549, C6 and NIH/3T3 whole cell lysates; Human fetal kidney and fetal brain lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IHC: Human kidney, human transitional cell carcinoma of bladder and mouse kidney tissues. ICC/IF: A549 cells. IP: A549 whole cell extract.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab179475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP 1/40.
ICC/IF 1/500.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD51 antibody
    • DKFZp686A08142 antibody
    • Integrin alpha five antibody
    • integrin alpha V beta 3 antibody
    • Integrin alpha-5 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • integrin alphaVbeta3 antibody
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • MSK 8 antibody
    • Msk8 antibody
    • Vitronectin receptor subunit alpha antibody
    • VNRA antibody
    • VTNR antibody
    see all

画像

  • All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution (8 hours at 4°C)

    All lanes : Mouse skin whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 116 kDa


    Exposure time: 25 seconds


    Diluent: 2% BSA in 1X TBST.

    Blocked using 5% BSA for 1 hour at RT.

     

    See Abreview

  • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green).

    Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).

    The negative controls are as follows:

    -ve control 1: ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

    Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: A549 whole cell extract.

    Lane 2: PBS instead of A549 whole cell extract.


    Blocking/Dilution buffer: 5% NFDM/TBST.

    ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  • All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Mouse spleen lysates
    Lane 4 : Rat brain lysates
    Lane 5 : Rat kidney lysates
    Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
    Lane 8 : Raji (Human Burkitt's lymphoma cell line) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 116 kDa
    Observed band size: 125,135 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  • Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

    Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution.

    Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysates
    Lane 2 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates
    Lane 3 : A549 (Human lung carcinoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 116 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  • All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysates
    Lane 2 : Human fetal brain lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 116 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

参考文献

This product has been referenced in:
  • Jessen TN & Jessen JR VANGL2 protein stability is regulated by integrin av and the extracellular matrix. Exp Cell Res 374:128-139 (2019). Read more (PubMed: 30472097) »
  • Shen M  et al. Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling. Cancer Cell 35:64-80.e7 (2019). IP ; Rabbit . Read more (PubMed: 30612941) »
See all 16 Publications for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human skin SCC)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Human skin SCC
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde

Mr. Yong Sun Lee

Verified customer

投稿 Jun 27 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse skin)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
40 µg
Specification
Mouse skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Mar 20 2018

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