Anti-Integrin alpha 9 抗体 [EPR9722] - BSA and Azide free (ab240116)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9722] to Integrin alpha 9 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Integrin alpha 9 antibody [EPR9722] - BSA and Azide free
Integrin alpha 9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9722] to Integrin alpha 9 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
ab240116 is the carrier-free version of ab140599.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9722 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab240116の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).
|
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
WB
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa). |
ターゲット情報
-
機能
Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. -
組織特異性
In airway epithelium, in the basal layer of squamous epithelium, and in smooth muscle, skeletal muscle, and hepatocytes. Abundantly expressed in fetal lung and lung cancers. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 3680 Human
- Entrez Gene: 104099 Mouse
- Entrez Gene: 685004 Rat
- Omim: 603963 Human
- SwissProt: Q13797 Human
- Unigene: 113157 Human
-
別名
- Alpha RLC antibody
- Integrin alpha 4 like antibody
- Integrin alpha RLC antibody
see all
画像
-
Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Integrin alpha 9 with ab140599 antibody at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140599).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Integrin alpha 9 with ab140599 antibody at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140599).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (1)
ab240116 は 1 報の論文で使用されています。
- Fu Y et al. Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting ß-Tubulin in Triple-Negative Breast Cancer. Onco Targets Ther 13:5323-5335 (2020). PubMed: 32606750