PE Anti-Integrin alpha 6 抗体 [GoH3] (ab95703)
Key features and details
- PE Rat monoclonal [GoH3] to Integrin alpha 6
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
PE Anti-Integrin alpha 6 antibody [GoH3]
Integrin alpha 6 一次抗体 製品一覧 -
製品の詳細
PE Rat monoclonal [GoH3] to Integrin alpha 6 -
由来種
Rat -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Integrin alpha 6. Mouse mammary tumor cells
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ポジティブ・コントロール
- Normal Human peripheral blood cells/leukocytes
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特記事項
The GoH3 antibody has been reported to have functional activity in blocking the binding of Integrin alpha 6 to laminin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
The GoH3 antibody has been reported to have functional activity in blocking the binding of Integrin alpha 6 to laminin. -
ポリ/モノ
モノクローナル -
クローン名
GoH3 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95703の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 0.06-0.125µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µl is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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特記事項 |
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Flow Cyt
Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能
Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). -
組織特異性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). -
関連疾患
Epidermolysis bullosa letalis, with pyloric atresia -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
翻訳後修飾
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
細胞内局在
Cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3655 Human
- Entrez Gene: 16403 Mouse
- Omim: 147556 Human
- SwissProt: P23229 Human
- SwissProt: Q61739 Mouse
- Unigene: 133397 Human
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別名
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
画像
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Flow Cytometry analysis of normal human peripheral blood cells stained with Rat IgG2a kappa Isotype Control Phycoerythrin (blue histogram) or Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703) (purple histogram). Cells in the lymphocyte gate were used for analysis.
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Flow cytometry staining of normal Human peripheral blood cells with 0.06 µg of Rat IgG2a Isotype Control PE (open histogram) or 0.06 µg of ab95703 (filled histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (11)
ab95703 は 11 報の論文で使用されています。
- Gerdes P et al. Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells. Nat Commun 13:7470 (2022). PubMed: 36463236
- Wang C et al. Elevated level of mitochondrial reactive oxygen species via fatty acid ß-oxidation in cancer stem cells promotes cancer metastasis by inducing epithelial-mesenchymal transition. Stem Cell Res Ther 10:175 (2019). PubMed: 31196164
- Lin X et al. Increased expression of TMED2 is an unfavorable prognostic factor in patients with breast cancer. Cancer Manag Res 11:2203-2214 (2019). PubMed: 31114314
- Krishna S et al. Dynamic expression of tRNA-derived small RNAs define cellular states. EMBO Rep 20:e47789 (2019). PubMed: 31267708
- Shabani R et al. Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(lactic-co-glycolic acid) nanoparticles in vitro. Int J Nanomedicine 13:2943-2954 (2018). PubMed: 29849458