Anti-Integrin alpha 6 抗体 [GoH3] (ab105669)
![Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (ab105669)](https://www.abcam.co.jp/ps/products/105/ab105669/Images/ab105669-118071-anti-integrin-alpha-6-antibody-goh3-flow-cytometry.gif "Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (ab105669)")
Key features and details
- Rat monoclonal [GoH3] to Integrin alpha 6
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-Integrin alpha 6 antibody [GoH3]
Integrin alpha 6 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [GoH3] to Integrin alpha 6 -
由来種
Rat -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Integrin alpha 6. Mouse mammary tumor cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
GoH3 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105669の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
| 特記事項 |
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Flow Cyt
Use at an assay dependent concentration. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
ターゲット情報
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機能
Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). -
組織特異性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). -
関連疾患
Epidermolysis bullosa letalis, with pyloric atresia -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
翻訳後修飾
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
細胞内局在
Cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3655 Human
- Omim: 147556 Human
- SwissProt: P23229 Human
- Unigene: 133397 Human
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別名
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
画像
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Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (ab105669)Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (open histogram)or 0.25 ug of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE Cells in the lymphocyte gate were used for analysis
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (11)
ab105669 は 11 報の論文で使用されています。
- Guo D et al. Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition. Development 147:N/A (2020). PubMed: 33144400
- Zhang K & Manninen A 3D Cell Culture Models of Epithelial Tissues. Methods Mol Biol 1926:77-84 (2019). PubMed: 30742264
- Tian J et al. Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation. Stem Cell Res Ther 10:382 (2019). PubMed: 31842987
- Day BW et al. The dystroglycan receptor maintains glioma stem cells in the vascular niche. Acta Neuropathol 138:1033-1052 (2019). PubMed: 31463571
- Storm RJ et al. Human Adenovirus Type 37 Uses aVß1 and a3ß1 Integrins for Infection of Human Corneal Cells. J Virol 91:N/A (2017). PubMed: 27974569
