Anti-Integrin alpha 3 抗体 [CL6937] (ab242196)
Key features and details
- Mouse monoclonal [CL6937] to Integrin alpha 3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Integrin alpha 3 antibody [CL6937]
Integrin alpha 3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CL6937] to Integrin alpha 3 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Integrin alpha 3.
Database link: P26006 -
ポジティブ・コントロール
- IHC-P: Human lung, colon and placenta tissue. WB: U-87 MG whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
CL6937 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab242196の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa.
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特記事項 |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa. |
ターゲット情報
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機能
Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. -
組織特異性
Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
翻訳後修飾
Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3675 Human
- Omim: 605025 Human
- SwissProt: P26006 Human
- Unigene: 265829 Human
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別名
- AA407068 antibody
- Antigen identified by monoclonal antibody J143 antibody
- CD49 antigen like family member C antibody
see all
画像
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Paraffin-embedded human colon tissue stained for Integrin alpha 3 using ab242196 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-Integrin alpha 3 antibody [CL6937] (ab242196) at 1 µg/ml
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells transfected with control siRNA
Lane 2 : U-87 MG cells transfected with target specific siRNA probe #1
Lane 3 : U-87 MG cells transfected with target specific siRNA probe #2
Predicted band size: 119 kDaRemaining relative intensity is presented.
Loading control: Anti-GAPDH
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Paraffin-embedded human placenta tissue stained for Integrin alpha 3 using ab242196 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human lung tissue stained for Integrin alpha 3 using ab242196 at 1/100 dilution in immunohistochemical analysis.
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Negative control:
Paraffin-embedded human liver tissue stained for Integrin alpha 3 using ab242196 at 1/100 dilution in immunohistochemical analysis.
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Anti-Integrin alpha 3 antibody [CL6937] (ab242196) at 1 µg/ml + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Predicted band size: 119 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab242196 は 3 報の論文で使用されています。
- Zhou J et al. Integrin α3/α6 and αV are implicated in ADAM15-activated FAK and EGFR signalling pathway individually and promote non-small-cell lung cancer progression. Cell Death Dis 13:486 (2022). PubMed: 35597804
- Zhu J et al. CD151 drives cancer progression depending on integrin a3ß1 through EGFR signaling in non-small cell lung cancer. J Exp Clin Cancer Res 40:192 (2021). PubMed: 34108040
- Zheng X et al. Cancer-Associated Fibroblasts Promote Vascular Invasion of Hepatocellular Carcinoma via Downregulating Decorin-integrin β1 Signaling. Front Cell Dev Biol 9:678670 (2021). PubMed: 34504839