Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Vessels)
Specification
Vessels
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pronase K
Permeabilization
No
Blocking step
Goat-Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Dilution
1/10
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: Dako Antibody Diluent
Secondary antibody
Name
Non-Abcam antibody was used: KPL
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Nov 06 2012