Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5789] to Integrin alpha 2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
製品名Anti-Integrin alpha 2 antibody [EPR5789] - BSA and Azide free
Integrin alpha 2 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5789] to Integrin alpha 2 - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,IHC-P or IP
Synthetic peptide within Human Integrin alpha 2 aa 1150-1250 (C terminal). The exact sequence is proprietary.
- WB: HeLa and A431 cell lysates.
ab247859 is the carrier-free version of ab109432 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247859 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab247859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 129 kDa.|
機能Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
配列類似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ITGA2 knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 129 kDa
Observed band size: 129 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab109432).
ab109432 Anti-Integrin alpha 2 antibody [EPR5789] was shown to specifically react with Integrin alpha 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264819 (knockout cell lysate ab257262) was used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.