Anti-Insulin Receptor beta 抗体 [18-44] (ab983)

Abreviews (1)Q&A (6)Specific References (5)

製品の概要

  • 製品名
    Anti-Insulin Receptor beta antibody [18-44]
    Insulin Receptor beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [18-44] to Insulin Receptor beta
  • 由来種
    Mouse
  • 特異性
    This antibody reacts specifically with the beta subunit of the insulin receptor.
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, Blocking, Functional Studies, IP, WBmore details
  • 種交差性
    交差種: Rabbit, Cow, Human
  • 免疫原

    Human placental insulin receptor.

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use at an assay dependent concentration. PubMed: 18411068
Blocking Use at an assay dependent concentration. Dilute in PBS or medium which is identical to that used in the assay system.
Functional Studies Use at an assay dependent concentration. Inhibition of insulin binding: IM-9 lymphocytes = 35%, Adipocytes = 90% Concentration for half maximal effect: Inhibition of insulin binding = 0.2nM, Stimulation of lipogenesis = 0.3nM. This antibody has insulin-like biological activity and therefore activates the receptor kinase.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Insulin receptor mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a ß-a-a-ß configuration. The ß-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kDa) is completely extracellular. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc.
  • 細胞内局在
    Membrane; Single pass type I membrane protein.
  • 参照データベース
    • 別名
      • CD220 antibody
      • HHF5 antibody
      • HIR B antibody
      • INSR antibody
      • Insulin receptor antibody
      • Insulin receptor subunit beta antibody
      • IR antibody
      see all

    画像

    • Flow Cytometry - Anti-Insulin Receptor beta antibody [18-44] (ab983)
      Flow Cytometry - Anti-Insulin Receptor beta antibody [18-44] (ab983)
      Overlay histogram showing HepG2 cells stained with ab983 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab983, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor beta antibody [18-44] (ab983)
      Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor beta antibody [18-44] (ab983)Image from Thiel CT et al., Mol Genet Metab. 2008 Jul;94(3):356-62. Epub 2008 Apr 14. Fig 4.; doi:10.1016/j.ymgme.2008.02.013; 14 April 2008, Molecular Genetics and Metabolism, 94 (3), 356–362, with permission from Elsevier.
      ab983, staining Insulin Receptor beta in Human skin fibroblast cells, by Immunocytochemistry/ Immunofluorescence.

      Fibroblasts cultured from skin biopsies of a patient with Rabson–Mendenhall syndrome and a healthy control, were fixed in methanol. Samples were fixed with primary antibody and a FITC-labeled donkey polyclonal mouse antibody (ab7057) was used as a secondary antibody. DNA was counterstained with DAPI.

    参考文献

    This product has been referenced in:
    • Yosten GL  et al. Evidence for an interaction between proinsulin C-peptide and GPR146. J Endocrinol 218:B1-8 (2013). ICC/IF . Read more (PubMed: 23759446) »
    • Thiel CT  et al. Two novel mutations in the insulin binding subunit of the insulin receptor gene without insulin binding impairment in a patient with Rabson-Mendenhall syndrome. Mol Genet Metab 94:356-62 (2008). ICC/IF ; Human . Read more (PubMed: 18411068) »

    See all 5 Publications for this product

    ab983 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    7 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-Insulin Receptor beta antibody [18-44]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Sample
    Mouse Tissue lysate - other (Hep G2)
    Specification
    Hep G2
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Jan 27 2014


    I would like to ask if you offer the insulin receptor antibody specific
    for exon 11 (aa 745-756). The goal should be to distinguish between IR-A
    and IR-B isoform of insulin receptor. Exon 11 is specific for IR-B
    isoform. Or if you o...

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    According to the Uniprot homepage the alpha chain is located from amino acid 28-758, whereas the beta chain is from aa 763-1382:

    http://www.uniprot.org/uniprot/P06213

    ab69508 has its immunogen in the cytoplasmic domain of ...

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    Post complaint survey:
    ...
    Q. Could we have done anything to better resolve the problem?
    A.The original CD63 antibody did not work despite the trying various permutations of blocking reagents etc. I requested an alternative antibody TSG1...

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    Thank you very much for having provided your feedback in the last survey.

    I am happy to hear that you were satisfied with our service and that the ab83 does work better than the original antibody received. I am sorry though, that the ab83 sti...

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    What is the epitope of this antibody? Do you know if it binds C-terminally?

    Thank you for your inquiry and your patience with this reply. We have limited data regarding the specific epitope for this antibody, but it appears that it is amino acids 765-770 (PEEHRP). I hope this information helps. Please contact us with an...

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    How are you all, love your team, anyways i have a customer which used your product and all failed on WB & IP. She would like to receive credit on this, can you credit our account? below is the protocol I had her email to me as i know this is required f...

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    Thanks for your email and I'm sorry to hear that your customer is experiencing difficulty with this antibody. I do require some additional details in order to investigate this. Exactly what type of samples were used? And from which species? How was...

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    I want to know how good the reactivity is with bovine (cow) species as it was mentioned in the product data sheet. Could you please send me any western blot pictures or any other data showing the reactivity of this antibody in bovine species? Thank...

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    Thank you for your enquiry. The reactivity of this antibody with bovine and rabbit insulin receptor is extremely weak. In Western blots, a very faint band can be seen in some samples, but this banding is not seen consistently in all samples. We menti...

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    What is the reactivity of the above antibody in bovine samples? On the datasheet it states "weak reactivity, but what does the WB look like and what dilution is needed. ?

    Thank you for your enquiry. This antibody produces very faint banding when used at relatively high concentration (25 ug/ml) to immunoblot bovine or rabbit insulin receptor. In contrast, immunoblotting on human insulin receptor produces a sharp, strong...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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