Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE - BSA and Azide free
- Suitable for: ICC, WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品名Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free
Insulin degrading enzyme / IDE 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE - BSA and Azide free
アプリケーション適用あり: ICC, WB, Flow Cytmore details
適用なし: IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 950-1050 (internal sequence). The exact sequence is proprietary.
Ab247897 is the carrier-free version of ab109538. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247897 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab247897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 118 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
機能Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
配列類似性Belongs to the peptidase M16 family.
翻訳後修飾The N-terminus is blocked.
細胞内局在Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
- Information by UniProt
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : Insulin degrading enzyme / IDE knockout HeLa cell lysate
Lane 3 : Wild-type Hap1 cell lysate
Lane 4 : Insulin degrading enzyme / IDE knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 118 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab109538).
ab109538 IDE antibody [EPR6099] was shown to specifically react with IDE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261755 (knockout cell lysate ab257197) was used. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab109538 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.