Anti-Insulin degrading enzyme / IDE 抗体 [EPR6099] - BSA and Azide free (ab247897)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free
Insulin degrading enzyme / IDE 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, HAP1, K562 and HepG2 cell lysates.
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特記事項
ab247897 is the carrier-free version of ab109538.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6099 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247897の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 118 kDa.
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 118 kDa. |
ターゲット情報
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機能
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. -
配列類似性
Belongs to the peptidase M16 family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. - Information by UniProt
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参照データベース
- Entrez Gene: 3416 Human
- Entrez Gene: 15925 Mouse
- Entrez Gene: 25700 Rat
- Omim: 146680 Human
- SwissProt: P14735 Human
- SwissProt: Q9JHR7 Mouse
- SwissProt: P35559 Rat
- Unigene: 500546 Human
see all -
別名
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
画像
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All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IDE knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab109538).
Lanes 1- 2: Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab109538 was shown to react with IDE in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261755 (knockout cell lysate ab257197) was used. Wild-type HeLa and IDE knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109538 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/2000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : IDE knockout HAP1 cell lysate,
Lane 3 : K562 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 118 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab109538).
Lanes 1 - 4: Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109538 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab109538 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247897 は論文での使用が確認できていません。