Application
ELISA
Sample
Mouse Purified protein (Pancreas)
Specification
Pancreas
Type
Sandwich (Detection)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 0.25% BSA
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse HRP IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Additional data
Additional Notes
ab6995 was used as a capture antibody, with ab14042 as a detection antibody.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in sandwich ELISA with ab14042 we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Vruti Patel
Verified customer
投稿 Nov 18 2014