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  1. Link

    insulin-antibody-e2e3-ab9569.pdf

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Signal Transduction Growth Factors/Hormones Insulin / Insulin-like
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Anti-Insulin 抗体 [E2E3] (ab9569)

  • Datasheet
  • SDS
Reviews (2)Q&A (11)References (2)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (ab9569)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (ab9569)

Key features and details

  • Mouse monoclonal [E2E3] to Insulin
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
Product image
Anti-IGF1 antibody (ab106836)
タンパク質
Product image
Recombinant human Insulin protein (Active) (ab123768)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-Insulin antibody [E2E3]
    Insulin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [E2E3] to Insulin
  • 由来種

    Mouse
  • 特異性

    The antibody recognizes the biologically most active forms of insulin on the C terminal end. The antibody labels the cytoplasm of beta cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method).
  • アプリケーション

    適用あり: IHC-Pmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Full length protein corresponding to Pig Insulin. Full length Native protein
    Database link: P01315

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    E2E3
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

関連製品

  • Affibody® Molecule

    • Anti-Insulin Affibody® Molecule (ab31906)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human Insulin protein (Active) (ab123768)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9569の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
1/100. Staining of formalin-fixed tissue sections requires treating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT.
特記事項
IHC-P
1/100. Staining of formalin-fixed tissue sections requires treating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT.

ターゲット情報

  • 機能

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 関連疾患

    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 配列類似性

    Belongs to the insulin family.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession P01308 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3630 Human
    • Omim: 176730 Human
    • SwissProt: P01308 Human
    • Unigene: 272259 Human
    • 別名

      • IDDM antibody
      • IDDM1 antibody
      • IDDM2 antibody
      • ILPR antibody
      • ins antibody
      • INS_HUMAN antibody
      • Insulin A chain antibody
      • Insulin B chain antibody
      • IRDN antibody
      • MODY10 antibody
      • Preproinsulin antibody
      • Proinsulin antibody
      • Proinsulin precursor antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (ab9569)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (ab9569)
      Human pancreas stained with ab9569.The antibody labels the cytoplasm of ß cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin embedded tissue sections using the Streptavidin-biotinylated peroxidase method).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (ab9569)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [E2E3] (ab9569)Image courtesy of an abreview from Nizar Mourad.

      Formalin-fixed, paraffin-embedded monkey pancreas tissue stained for Insulin using ab9569 at 1/800 dilution in immunhistochemical analysis.

      Goat Ani-mouse HRP was used as the secondary antibody.

    プロトコール

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (2)

    ab9569 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9569 は 2 報の論文で使用されています。

    • Tian M  et al. Alternative immunomodulatory strategies for xenotransplantation: CD80/CD86-CTLA4 pathway-modified immature dendritic cells promote xenograft survival. PLoS One 8:e69640 (2013). IHC-P . PubMed: 23922766
    • Nykiforuk CL  et al. Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds. Plant Biotechnol J 4:77-85 (2006). WB ; Human . PubMed: 17177787

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Insulin antibody [E2E3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Pancreas)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Pancreas
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Nizar Mourad

    Verified customer

    投稿 Apr 19 2016

    Western blot abreview for Anti-Insulin antibody [E2E3]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Arabidopsis thaliana Recombinant protein (total seed extract)
    Specification
    total seed extract
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Dr. Cory L Nykiforuk

    Verified customer

    投稿 Feb 24 2006

    Question

    Would like to use this antibody to target a commercially available insulin analog, becausethis antibodydoesn't react with rat insulin.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 19 2012

    Answer

    Thank you very much for your call today and for your enquiry.

    As we discussed, please keep me updated about the results with this antibody. Should you have any trouble with it, I will be happy to set up the testing program for you in retrospect.

    Please let me know if you have any questions or if there is anything else that we can do for you. Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Sep 19 2012

    Question

    Todayi aliquot anti- insulin antibody (ab9569) and wrongly stored it at -20°C. now is this antibody is usable? or destroyed?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 09 2012

    Answer

    Thank you for your enquiry.
    The antibody should be OK. I would recommend using the antibody in an experiment using a positive control sample to make sure it is still active.
    Store remainder of antibody at 4 degrees C.
    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 09 2012

    Question

    I want to measure monmeric insulin and hexameric form of Insulin in serum separately . I need antibodies to distinguish them in western or ELISA method. Which one of your antibody products can help me?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2012

    Answer

    Thank you for contacting us. Because the sequence is conserved between the monomeric and hexameric forms of insulin, it would not be possible to get antibodies that would be specific for one form over the other. The easiest way to tell the difference between the two forms would be by their molecular weights in a native WB. For this, I would recommend either ab14042 or ab9569. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Jun 27 2012

    Question

    Have you tested this antibody in WB?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 23 2012

    Answer

    Thank you for your inquiry and I apologize for the delay in this reply.


    We do not test this antibody in WB in-house. The only information we have about the use of this antibody is from customer data in the form of an Abreview and reference.


    https://www.abcam.com/Insulin-antibody-E2E3-ab9569.html&tab=abreviews&intabreviewid=4206


    https://www.abcam.com/Insulin-antibody-E2E3-ab9569-references.html


    If you're having difficulty with this antibody in WB, please let us know by providing some protocol information:


    1) Abcam order reference number or product batch number

    2) Description of the problem

    3) Sample preparation:

    Type of sample (whole cell lysates, fraction, recombinant protein…):

    Lysis buffer

    Protease inhibitors:

    Phosphatase inhibitors

    Reducing agent

    Boiling for ≥5 min? yes/no

    Protein loaded ug/lane or cells/lane

    Positive control

    Negative control

    4) Percentage of gel

    Type of membrane

    Protein transfer verified

    Blocking agent and concentration

    Blocking time

    Blocking temperature

    5) Primary antibody (If more than one was used, describe in “additional notes”) :

    Concentration or dilution

    Diluent buffer

    Incubation time

    Incubation temperature:

    6) Secondary antibody:

    Species:

    Reacts against:

    Concentration or dilution

    Diluent buffer

    Incubation time

    Incubation temperature:

    Fluorochrome or enzyme conjugate:

    7) Washing after primary and secondary antibodies:

    Buffer

    Number of washes

    8)Detection method

    9) How many times have you run this staining?

    Do you obtain the same results every time?

    What steps have you altered to try and optimize the use of this antibody?

    Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.


    With this information, we will be able to assess any difficulties with the product and offer a replacement or refund if necessary in accordance with our Abpromise. I hope this information helps. Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Jan 23 2012

    Question

     Dear Colleagues from Abcam,     My customer would like to order antibodies: - Anti-Insulin antibody [E2E3] (ab9569) - Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) On the website there is information that they were tested in Western Blotting. My customer would like to know what were the Western Blotting electrophoresis conditions – native or denaturing - in which they were tested? She has a problem with WB, the anti-proinsulin antibody doesn’t bind the proinsulin and she doesn’t know what she is doing wrong.   I would be grateful for your help.   Best regards,    

    Read More

    Abcam community

    Verified customer

    Asked on Nov 23 2011

    Answer

    Thank you for your inquiry. Please excuse the delay of my answer, but I am contacting the source of ab8304 and haven't heard back from them yet. But I am happy to confirm that ab9569 will weakly detect non-reduced insulin (needed to load at least 0.5 ug of purified protein). Also detected reduced or non-reduced proinsulin weakly (need to load at least 0.25 ug of purified protein). I therefore suggest to use reducing condition with this antibody and also recommend to load enough protein. Please excuse the wait, I will get back to you as soon as I receive more information about ab8304.

    Read More

    Abcam Scientific Support

    Answered on Nov 23 2011

    Question

    Customer experiencing difficulties using ab9569 in WB.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 11 2011

    Answer

    According to our records, ab9569 was proving difficult to use in WB and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution. If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records. We wish you the best of luck with your research and look forward to a reply.  

    Read More

    Abcam Scientific Support

    Answered on Nov 11 2011

    Question

    I want to know the insulin antibody (ab7842, ab14042, ab9569) which one can be used to detect ng level insulin? or which one has better sensitivity?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2008

    Answer

    Thank you for your enquiry. I apologize for the delay in replying to you. It has taken some time to gather all the required information. Sensitivity of antibodies in ELISA assays depends very much on the individual assay procedure and the type of samples. However, in testing I can confirm that the sensitivity of ab9569 Insulin antibody [E2E3] is between 1.0 to 10.0 ng/ml, and the detection level for ab14042 Insulin antibody appears to be ~1 ng/ml. I am sorry we have no further data regarding the minimum detection limits of ab7842 Insulin antibody I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Oct 10 2008

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal... SAMPLE Porcine Insulin from pancreas (whole molecule) PRIMARY ANTIBODY Capture antibody: ab9569 diluted in phoshate buffer (50mM) diluted to 2 microgram/mL. Incubated first time 2 hours 37C, second time overnight 4C. DETECTION METHOD Conjugated ab used: ab6877. Diluted to manufacturers rules 1:4000. Works well in other tests. POSITIVE AND NEGATIVE CONTROLS USED As a blank I followed the same procedure as mentioned above, but left the antigen incubation behind. A positive control I couldn't perform, because I'm setting this assay up from zero. So the positive control would be this experiment :-} ANTIBODY STORAGE CONDITIONS 4 degrees Celsius TYPE OF ELISA Sandwich ELISA COATING WELL Buffer: Sodium phosphate buffer (50mM) Concentration coating MAb (ab9569): 2 microg/mL BLOCKING CONDITIONS PBS with 1%BSA, for 2 hours at 37 degrees Celsius SECONDARY ANTIBODY Detection antibody: ab14042 diluted in blocking buffer (see above) diluted to 1 microgram/mL. Incubated first time 2 hours 37C, second time overnight 4C (used 2 methods: 1. incubation first with antigen and then added secondary ab, 2. incubation at the same time with antigen). Both methods gave no signal HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? incubation time/temperature of 1st antibody, antigen and 2nd antibody

    Read More

    Abcam community

    Verified customer

    Asked on May 10 2006

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and you are largely following an ELISA protocol that we would recommend. There is no reason why these antibodies would not work together in a sandwich ELISA approach. However, we recommend that the capture antibody is seeded at a concentration of 1-10ugml-1. I therefore consider it a useful exercise to increase the concentration of ab9569 to fully determine whether this antibody is not working by ELISA. It may also be useful to seed a plate with the porcine insulin antigen itself and perform a direct approach using an anti-mouse secondary antibody to determine whether it is the antibody or the combinations of antibodies to blame. We have a direct ELISA protocol available at the following link: https://www.abcam.com/assets/pdf/protocols/Direct%20ELISA%20protocol.pdf I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 12 2006

    1-10 of 13 Abreviews or Q&A

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