Anti-Insulin + Proinsulin 抗体 [D6C4] (ab8304)

製品の概要

  • 製品名
    Anti-Insulin + Proinsulin antibody [D6C4]
    Insulin + Proinsulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [D6C4] to Insulin + Proinsulin
  • 由来種
    Mouse
  • 特異性
    This antibody is specific for insulin and proinsulin.
  • アプリケーション
    適用あり: Electron Microscopy, IP, ELISA, IHC-Fr, ICC/IF, Sandwich ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Pig
  • 免疫原

    Human insulin.

  • 特記事項

    Kd for this antibody is 8.1 x 10-8M.

    Detection of both insulin and proinsulin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Electron Microscopy Use at an assay dependent concentration. PubMed: 19628478
IP 1/100.
ELISA Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 24614166
Sandwich ELISA Use a concentration of 10 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [D3E7] to Insulin + Proinsulin (Biotin) (ab20756) and Mouse monoclonal [D3E7] to Insulin + Proinsulin (HRP) (ab28063). Use at a concentration of 10 µg/ml, 50µL per well. Can be used as Capture antibody with recommended pair(s).
IHC-P 1/1250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

画像

  • ab8304 at 1/1250 staining developing mouse pancreas tissue sections (E14/15) by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in Tris buffer was performed. The tissue was then blocked and incubated with the antibody for 2 hours. A biotinylated goat anti-mouse IgG antibody was used as the secondary.

    See Abreview

  • ab8304 used on human pancreas. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in Tris buffer was performed. The tissue was then blocked and incubated with the antibody for 2 hours. A biotinylated goat anti-mouse IgG antibody was used as the secondary.

    See Abreview

参考文献

This product has been referenced in:
  • Kim J  et al. HX-1171 attenuates pancreatic ß-cell apoptosis and hyperglycemia-mediated oxidative stress via Nrf2 activation in streptozotocin-induced diabetic model. Oncotarget 9:24260-24271 (2018). Read more (PubMed: 29849938) »
  • Mor F  et al. Diabetogenic Effects of Ochratoxin A in Female Rats. Toxins (Basel) 9:N/A (2017). IHC-P ; Rat . Read more (PubMed: 28422066) »

See all 11 Publications for this product

レビューと Q&A

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (pancreatic islet cells)
Loading amount
50 µg
Specification
pancreatic islet cells
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Sarah Wang

Verified customer

投稿 May 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MIN6 cell line)
Total protein in input
600 µg
Specification
MIN6 cell line
Treatment
RIPA buffer with SDS for 5 min
Immuno-precipitation step
Protein A
Username

Yuichi Tsuchiya

Verified customer

投稿 Jul 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (developing pancreas E14/15)
Specification
developing pancreas E14/15
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: I0mM TRIS pH10
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Jun 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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