Application
Western blot
Sample
Mouse Cell lysate - whole cell (HL-1 cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (4-12 %)
Loading amount
40 µg
Treatment
LPS 1 µg/ml
Specification
HL-1 cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 130 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Barbara Thaler
Verified customer
投稿 Jul 03 2019