Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Ovary (abnormal))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween 20, pH 6.0
Permeabilization
No
Specification
Ovary (abnormal)
Blocking step
ab127055 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS
Dilution
1/50
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Immunohistochemical analysis of formalin fixed, paraffin embedded abnormal mouse ovary tissue, labeling inhibin alpha with ab14087. Following antigen retrieval in 10mM Sodium Citrate, 0.05% Tween 20, pH 6.0. Mouse on Mouse Polymer IHC Kit. Visualization was with DAB substrate and counter-stained with hematoxylin.
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投稿 Aug 17 2016