Name: Anti-Inhibin alpha antibody [R1]
SKU: ab14087
Rating: 5 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Key features and details

Mouse monoclonal [R1] to Inhibin alpha
Suitable for: IHC-P
Reacts with: Human
Isotype: IgG2a

製品名

Anti-Inhibin alpha antibody [R1]
Inhibin alpha 一次抗体製品一覧
製品の詳細

Mouse monoclonal [R1] to Inhibin alpha
由来種

Mouse
アプリケーション

適用あり: IHC-Pmore details
種交差性

交差種: Human
免疫原

Synthetic peptide corresponding to Human Inhibin alpha aa 233-264. Note that this peptide corresponds to the N-terminus of the mature region of the human inhibin alpha subunit.
Sequence:
STPLMSWPWSPSALRLLQRPPEEPAAHANCHR

Database link: P05111
Run BLAST with Run BLAST with
ポジティブ･コントロール
- human ovary, testis, placenta
特記事項

Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.08% Sodium azide
Constituent: Tissue culture supernatant
Concentration information loading...
精製度

Tissue culture supernatant
一次抗体備考

Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.
ポリ/モノ

モノクローナル
クローン名

R1
アイソタイプ

IgG2a
研究分野

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
Recombinant Protein
- Recombinant Human Inhibin alpha protein (ab53378)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab14087の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P	(1)	1/1 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Neat - 1/1/50. Sodium citrate pH 6.0 is recommended for antigen retrieval.

特記事項
IHC-P 1/1 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Neat - 1/1/50. Sodium citrate pH 6.0 is recommended for antigen retrieval.

機能

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
組織特異性

Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
配列類似性

Belongs to the TGF-beta family.
翻訳後修飾

Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
細胞内局在

Secreted.
Target information above from: UniProt accession P05111 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 3623 Human
- Omim: 147380 Human
- SwissProt: P05111 Human
- Unigene: 407506 Human
別名
- A inhibin subunit antibody
- A inhibin subunit precursor antibody
- AW55578 antibody
- IHA antibody
- inhA antibody
- INHA_HUMAN antibody
- Inhibin alpha chain antibody
- Inhibin alpha chain precursor antibody
- Inhibin alpha subunit antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Immunohistochemical analysis of paraffin embedded human ovary tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H₂O₂ and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue (showing sertoli cells, leydig cells and spermatogonia) labeling inhibin alpha with ab14087.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H₂O₂ and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H₂O₂ and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [R1] (ab14087)

Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

Immunohistochemistry protocols

Click here to view the general protocols

SDS download

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ab14087 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14087 は 4 報の論文で使用されています。

Kusumoto S et al. An immature inhibin-a-expressing subpopulation of ovarian clear cell carcinoma cells is related to an unfavorable prognosis. Cancer Med 10:1485-1500 (2021). PubMed: 33611864
Walton CC et al. Primary neurons can enter M-phase. Sci Rep 9:4594 (2019). PubMed: 30872646
Xu Y et al. Cystic and solitary nodular pulmonary metastases in a patient with low-grade endometrial stromal sarcoma: A case report and literature review. Oncol Lett 18:1133-1144 (2019). PubMed: 31423173
Chen J et al. Computed tomography and magnetic resonance imaging features of ovarian fibrothecoma. Oncol Lett 14:1172-1178 (2017). PubMed: 28693292

レビューと Q&A

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Ovary (abnormal))

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween 20, pH 6.0

Permeabilization

Specification

Ovary (abnormal)

Blocking step

ab127055 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 21°C

Fixative

Formaldehyde

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投稿 Aug 17 2016

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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

一次抗体 備考

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (4)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Inhibin alpha antibody [R1]

一次抗体備考