Anti-ING4 抗体 (ab3714)
Key features and details
- Goat polyclonal to ING4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ING4 antibody
ING4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ING4 -
由来種
Goat -
特異性
Tested on a U2OS cell line overexpressing human ING4. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog -
免疫原
Synthetic peptide:
CLVRTSPEYGMPS
, corresponding to amino acids 163 - 174 of Human ING4. -
特記事項
ING4 is moderately similar to ING1 and may act in chromatin-mediated transcriptional regulation. ING4 contains a PHD-finger.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
ING4 is moderately similar to ING1 and may act in chromatin-mediated transcriptional regulation. ING4 contains a PHD-finger. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3714の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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特記事項 |
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WB
1/500 - 1/3000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
ターゲット情報
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機能
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). -
配列類似性
Belongs to the ING family.
Contains 1 PHD-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 51147 Human
- Entrez Gene: 28019 Mouse
- Entrez Gene: 297597 Rat
- Omim: 608524 Human
- SwissProt: Q9UNL4 Human
- SwissProt: Q8C0D7 Mouse
- Unigene: 524210 Human
- Unigene: 262547 Mouse
see all -
別名
- Brain my036 protein antibody
- Candidate tumor suppressor p33 ING 1 homolog antibody
- Candidate tumor suppressor p33 ING1 homolog antibody
see all
画像
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Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot. This antibody was used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at ~29 kDa corresponding to ING4.
A 4-20% Tris-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rabbit anti Goat IgG [H&L] for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates. This antibody was used at 1.0 µg/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells. Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates. This antibody was used at 1.0 ug/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab3714 は 7 報の論文で使用されています。
- Culurgioni S et al. Crystal structure of inhibitor of growth 4 (ING4) dimerization domain reveals functional organization of ING family of chromatin-binding proteins. J Biol Chem 287:10876-84 (2012). WB . PubMed: 22334692
- Conner J & Braidwood L Expression of inhibitor of growth 4 by HSV1716 improves oncolytic potency and enhances efficacy. Cancer Gene Ther 19:499-507 (2012). WB . PubMed: 22595793
- Moreno A et al. Functional impact of cancer-associated mutations in the tumor suppressor protein ING4. Carcinogenesis 31:1932-8 (2010). WB ; Human . PubMed: 20705953
- Li X et al. Inhibitor of growth 4 induces growth suppression and apoptosis in glioma U87MG. Pathobiology 76:181-92 (2009). PubMed: 19571607
- Li X et al. ING4 induces cell growth inhibition in human lung adenocarcinoma A549 cells by means of Wnt-1/beta-catenin signaling pathway. Anat Rec (Hoboken) 291:593-600 (2008). PubMed: 18399550
- Iizuka M et al. Hbo1 Links p53-dependent stress signaling to DNA replication licensing. Mol Cell Biol 28:140-53 (2008). WB ; Human . PubMed: 17954561
- Nozell S et al. The ING4 tumor suppressor attenuates NF-kappaB activity at the promoters of target genes. Mol Cell Biol 28:6632-45 (2008). WB ; Human . PubMed: 18779315