Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, epithelial cells)
Specification
kidney, epithelial cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C · Diluent: 1%FCS PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti-goat alexafluor 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Maria Amorim
Verified customer
投稿 Apr 03 2012