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Anti-Influenza A Virus Nucleoprotein 抗体 [AA5H] (ab20343)

Reviews (2)Q&A (5)References (82)
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

Key features and details

  • Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Influenza A
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Influenza A Virus Nucleoprotein antibody [AA5H]
    Influenza A Virus Nucleoprotein 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Influenza A

  • 免疫原

    Tissue, cells or virus corresponding to Influenza A Influenza A Virus Nucleoprotein. Influenza A/Puerto Rico/8/34 (H1N1) and A/Bangkok/1/79 (H3N2) viruses.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    >95% pure (SDS-PAGE).

  • ポリ/モノ

    モノクローナル

  • クローン名

    AA5H

  • ミエローマ

    P3x63-Ag8.653

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab20343の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/1000.
特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/1000.

ターゲット情報

  • 関連性

    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

  • 細胞内局在

    Host cell nucleus

  • 参照データベース

    • 別名

      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

      Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.

      This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)Image from Dr RK Nelli at al, BMC Vet Res. 2010 Jan 27;6:4, Fig 5.
      ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
      Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fa
    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)Image is courtesy of an AbReview submitted by Dr Maria Amorim.

      Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.

      See Abreview

    参考文献 (82)

    ab20343 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab20343 は 82 報の論文で使用されています。

    • Pezzotti G  et al. Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses. Sci Rep 11:2977 (2021). PubMed: 33536558
    • Margaroli C  et al. Spatial mapping of SARS-CoV-2 and H1N1 lung injury identifies differential transcriptional signatures. Cell Rep Med 2:100242 (2021). PubMed: 33778787
    • Wu X  et al. Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. Cell Chem Biol 27:571-585.e6 (2020). PubMed: 32243810
    • Xia S  et al. Coupled CRC 2D and ALI 3D Cultures Express Receptors of Emerging Viruses and Are More Suitable for the Study of Viral Infections Compared to Conventional Cell Lines. Stem Cells Int 2020:2421689 (2020). PubMed: 32695180
    • Wang WC  et al. The Impacts of Reassortant Avian Influenza H5N2 Virus NS1 Proteins on Viral Compatibility and Regulation of Immune Responses. Front Microbiol 11:280 (2020). PubMed: 32226416
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Influenza A Virus Nucleoprotein antibody [AA5H]

     Excellent
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Dog Cultured Cells (MDCK)
    Specification
    MDCK
    Fixative
    Methanol
    Permeabilization
    Yes - Acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 30 2010

    Immunocytochemistry abreview for Anti-Influenza A Virus Nucleoprotein antibody [AA5H]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell lysate - whole cell (influenza A virus infected cells)
    Specification
    influenza A virus infected cells
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
    Read More

    Dr. Paul Digard

    Verified customer

    投稿 Aug 24 2005

    Question

    I am trying to do immunoprecipitation with one of your influenza virus antibodies. Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343) I have read your online protocol for immunoprecipitation https://www.abcam.com/ps/pdf/protocols/Immunoprecipitation%20protocol%20(IP).pdf and I have a doubt, at the point immunoprecipitation: Point 7. Finally, remove the last supernatant and add 25-50 μl of 2x loading buffer. Boil at 95-100°C for 5 minutes to denature the protein and separate it from the protein-A/G beads, then centrifuge and keep the supernatant where the protein is now. Once I do the elution in 2x loading buffer, do I need to further dilute the sample with distilled water 1 in 2 before loading the sample into the gel? Otherwise I will be loading the samples in 2x loading buffer..... Thank you very much for your help in advance!

    Read More
    Answer

    You will not need to dilute your sample any further after eluting from the beads. As the protocol says, you can after boiling load your sample directly onto your gel, without it affecting anything. If there is anything else I can help you with, please let me know.

    Read More

    Question

    I’ve been using the influenza A nucleoprotein antibody [AA5H] (ab20343) to stain for influenza A PR/8 (H1N1) infected cells. It’s worked great so far. However, I haven’t had success in staining cells (MDCK) with influenza A X-31 (H3N2). According to the specs, AA5H should work with H3N2 subtype. Was wondering if abCam has any information or insight on using AA5H for the X31 strain. I’ve also tried primary antibody 431-FITC on X31; however, I have not had success with that either.

    Read More
    Answer

    Thanks for your inquiry. The monoclonal antibody to Influenza A Nucleoprotein, catalog ab20343, has been tested and is reactive with the following Influenza A strains: H1N1 A/Puerto Rico/8/34A/USSR/90/77 A/England/333/80 A/Chile/1/83 A/Dunedin/6/83 A/Victoria/7/83 H2N2 A/Ann Arbor/6/60 H3N2 A/Aichi/2/68 A/Port Chambers/1/73 A/Bangkok/2/79 A/Korea/1/82 A/Philippines/2/82 A/Caen/1/84 A/Pennsylvania/27/84 For ab81126 : we used a vaccine as the immunogen of ab81126, and used the recombinant protein NP to screen positive clones. So the antibody of ab81126 can be used to detect the NP protein. We are not sure whether this antibody can react with all strains of influenza A virus, because we haven't done the relating tests. I hope this was helpful. Please feel free to contact me if you require further assistance.

    Read More

    Question

    Can this antibody detect intact influenza A virus?

    Read More
    Answer

    Thank you for your inquiry. The laboratory confirmed that they have not tested ab20343 against the intact virus.  The following link is to a published reference that describes the development and original characterizaton of this clone.  The paper includes IFA testing of cells infected with viruses reisolated from clinical Influenza A specimens. http://jcm.asm.org/cgi/reprint/23/2/240 Catalog ab20343, Clone AA5H, is described as Clone A1 in the paper.  I hope this information helps. Please contact me with any other questions.

    Read More

    Question

    My post doc would like to know if you have available the specific recognition sequence for this antibody.

    Read More
    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab20343 (Influenza A Virus Nucleoprotein antibody [AA5H]). All the characterization information we have on this product is from the following published reference: http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=268620&blobtype=pdf Note that Clone AA5H is Clone A1 in the paper. I hope this information helps. Please don't hesitate to contact us again if you have further questions.

    Read More

    Question

    Your company offers a monoclonal antibody against influenza A-Nucleoprotein. It is offered for the application in immunocytochemistry. My question is: Is it possible to use this antibody in immunohistochemistry in formalin fixed and paraffin embedded tissue? Or only in cryostat sections?

    Read More
    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in either formalin-fixed paraffin-embedded or frozen sections. It has been tested in immunocytochemistry and immunofluorescence in cultured cells. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

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