Anti-Influenza A Virus M2 Protein 抗体 [14C2] (ab5416)

製品の概要

  • 製品名
    Anti-Influenza A Virus M2 Protein antibody [14C2]
    Influenza A Virus M2 Protein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
  • 由来種
    Mouse
  • アプリケーション
    適用あり: Inhibition Assay, Flow Cyt, ICC/IF, ICC, IP, WBmore details
  • 免疫原

    Tissue, cells or virus corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.

  • エピトープ
    Detects the N-terminal of the Influenza A Virus M2 Protein.
  • ポジティブ・コントロール
    • ICC/IF: Infected MDCK cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
  • ポリ/モノ
    モノクローナル
  • クローン名
    14C2
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Inhibition Assay Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa.

ターゲット情報

  • 関連性
    The Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
  • 細胞内局在
    Virion membrane. Apical cell membrane; Single-pass type III membrane protein.
  • 別名
    • Influenza A virus matrix protein M2 antibody
    • M antibody
    • M2 antibody
    • Matrix protein 2 antibody
    • Membrane ion channel M2 antibody
    • Membrane protein M2 antibody
    • Proton channel protein M2 antibody
    see all

画像

  • Immunofluorescence staining of infected MDCK cells using ab5416.

参考文献

This product has been referenced in:
  • Tutykhina I  et al. Vaccination potential of B and T epitope-enriched NP and M2 against Influenza A viruses from different clades and hosts. PLoS One 13:e0191574 (2018). Read more (PubMed: 29377916) »
  • Opriessnig T  et al. Comparison of the efficacy of a commercial inactivated influenza A/H1N1/pdm09 virus (pH1N1) vaccine and two experimental M2e-based vaccines against pH1N1 challenge in the growing pig model. PLoS One 13:e0191739 (2018). WB . Read more (PubMed: 29381710) »

See all 33 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (influnza A infected)
Specification
influnza A infected
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Miss. natalie blair

Verified customer

投稿 Jun 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (M2 transfected PG13's)
Specification
M2 transfected PG13's
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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